Manuel
Condinho Carvalho
...
DP_AEM student
Instituto de Tecnologia Química e Biológica (ITQB)
Doctoral Program in Molecular Biosciences (ITQB)
2018-2022
Cecília Arraiano
Manuel
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Phd Thesis

Exploring the role of RNA degradation mechanisms in control of gene expression - PD/BD/150602/2020

Small non-coding RNAS (sRNAs) and ribonucleases (RNases) are two important players in gene expression regulation, being involved in Escherichia coli stress response. A common response mechanism to stress is the formation of biofilm. In our laboratory, it has been observed that, in E. coli, the three main exoribonucleases (RNase II, RNase R and PNPase) and some sRNAs influence the formation of biofilm and stress response. Previous results have shown that, compared to the wild type, the RNase II and RNase R deletion mutants produce more biofilm, while the PNPase deletion mutant is not able to form biofilm. We think this occurs because exoribonucleases may be indirectly affecting the levels of c-di-GMP, a metabolite that controls bacterial motility. Additionally, it has also been found that RNase II appears to be necessary for the response to acidic stress, since the strain deficient in this exoribonuclease is unable to grow after acidic shock. This mutant also has a significantly reduced expression of ArrS, a sRNA that is upregulated during the acidic response. Given this, we aim to describe the role of RNase II in the expression of ArrS and characterize the role of this sRNA in the response to acidic shock. Another sRNA that is altered in the RNase II mutant is FnrS, a sRNA involved in anaerobic respiration. The expression of FnrS is abnormally increased in RNase II and RNase R mutants, during aerobic conditions. We hypothesize that RNase II and RNase R have a role in the regulation of FnrS expression, namely by promoting its degradation in aerobiosis. Given this, we aim to describe the role of these exoribonucleases in the expression of ArrS and FnrS sRNAs, and understand how E. coli exoribonucleases regulate stress response, namely by influencing the formation of biofilm and the response to acidic shock and anaerobiosis.

Publication and Meetings

Alves, I.T.S., Condinho, M., Custódio, S. et al. (2018) Genetics of personalized medicine: cancer and rare diseases. Cellular Oncology, 41(3), 335-341.

 

16/03/2021 (Online) - Exploring the role of RNA degradation mechanisms in control of gene expression

21st Annual Meeting of the Portuguese Society of Human Genetics (2017)

Rare Diseases Symposium, INSA (2017)

 2nd International Meeting of the Portuguese Society of Genetics, Online (July, 2021)

Microbiotec Conference, Online (November, 2021)

Poster Communication:

Vânia Pobre, Manuel Condinho, Fábio Santos and Cecília M. Arraiano. Investigating how exoribonucleases and small RNAs influence bacterial adaptation to stress. 26th Annual Meeting of the RNA Socienty, Online, May 25 - June 4, 2021

Manuel Condinho, Fábio Santos, Cecília M. Arraiano and Vânia Pobre. Influence of exoribonucleases on small RNAs expression and bacterial stress response. 2nd International Meeting of the Portuguese Society of Genetics, Online (July, 2021)

Manuel Condinho, Fábio Santos, Cecília M. Arraiano and Vânia Pobre. Investigating how exoribonucleases and small RNAs influence bacterial adaptation to stress. Microbiotec Conference, Online (November, 2021)