Cátia
Pereira
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2016-2020
Manuela Côrte-Real
Cátia
0
0

Phd Thesis

Novel insights on the interaction of lactoferrin with proton pumping ATPases towards understanding its antifungal and anticancer activities

Lactoferrin (Lf) is a naturally occurring iron-binding protein and one of the most well-known milk bioactive compounds. In addition to milk, it is also found in different fluids of mammals including several mucosal surfaces. A multitude of biological activities, such as antimicrobial, antiviral, immunomodulatory and anticancer, have been ascribed to this protein, revealing its multifunctional character. Among them, its antifungal and anticancer activities stand out, as toxicity against a wide collection of yeasts and filamentous fungi, and a broad range of cancer types has been demonstrated. However, the molecular mechanisms underlying these activities are still poorly elucidated, which limits Lf applications as an anticancer and antifungal agent. In the present thesis, we sought to improve the current knowledge on the mechanisms through which Lf exerts its cytotoxicity against fungal and cancer cells, aiming to contribute to its rational, targeted and more efficient application. 

 

Supervisors:

Manuela Côrte-Real (CBMA, University of Minho)

Lígia Rodrigues (CEB, University of Minho)

 

Thesis External Advisor (CAT): Maria José Oliveira (Institute for Research and Innovation in Health, i3S)

 

PhD Fellowsip: PD/BD/128032/2016 (01/09/2016 - 14/09/2021)

University of Minho | PhD program on Molecular and Environmental Biology

PhD awarded with "Muito Bom" (highest distinction) by University of Minho (September 14, 2021)

 

 

Publication and Meetings

Peer-Reviewed Articles:

Santos-Pereira C, Rocha JF, Fernandes HS, Rodrigues LR, Côrte-Real M, Sousa SF (2021) The milk-derived lactoferrin inhibits V-ATPase activity by targeting its V1 domain. Int J Biol Macromol. 5, 186:54-70.  doi: 10.1016/j.ijbiomac.2021.06.200.

 

Chaves SR, Rego A, Martins VM, Santos-Pereira C, Sousa MJ, Côrte-Real M (2021) Regulation of cell death induced by acetic acid in yeasts. Frontiers in Cell and Developmental Biology. Front Cell Dev Biol. 9: 642375. doi: 10.3389/fcell.2021.642375

 

Santos-Pereira C, Rodrigues LR, Côrte-Real M (2021) Emerging insights on the role of V-ATPase in human diseases: therapeutic challenges and opportunities. Medicinal Research Reviews. 41(4):1927-1964. doi: 10.1002/med.21782

 

Santos-Pereira C, Andrés MT, Chaves SR, Fierro JF, Gerós H, Manon S, Rodrigues LR, Côrte-Real M (2021) Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiaeInternational Journal of Biological Macromolecules. 171: 343-357. doi: 10.1016/j.ijbiomac.2020.12.224

 

Pereira AC, Ferreira D, Santos-Pereira C, Vieira TF, Sousa SF, Sales G, Rodrígues LR (2020) Selection of a new peptide homing SK-BR-3 breast cancer cells. Chemical Biology & Drug Design. 97(4):893-903. doi: 10.1111/cbdd.13816

 

Barbosa C*Santos-Pereira C*, Soares I, Martins V, Terra-Matos J, Côrte-Real M, Lúcio M, Oliveira MECDR, Gerós H (2019) Resveratrol-loaded nanoformulations are internalized by endocytosis in yeast. Journal of Natural Products. 82, 5, 1240-1249. doi: 10.1021/acs.jnatprod.8b01003 *Both authors contributed equally

 

Costa D, Borges J, Mota MF, Rodrigues MS, Pereira-Silva P, Ferreira A, Pereira CS, Sampaio P, Vaz F (2019) Effect of microstructural changes in the biological behavior of magnetron sputtered ZnO thin films. Journal of Vacuum Science and Technology. 37, 011501. doi: 10.1116/1.5048785

 

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M (2018) Bovine Milk Lactoferrin Selectively Kills Highly Metastatic Prostate Cancer PC-3 and Osteosarcoma MG-63 Cells In Vitro. Frontiers in Oncology. 8:200. doi: 10.3389/fonc.2018.00200

 

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. 7(38):62144-62158. doi: 10.18632/oncotarget.11394

 

Acosta-Zaldívar M,Andrés MT, Rego A, Pereira CS, Fierro JF, Côrte-Real M (2016) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiae. Apoptosis. 21(2):163-73. doi: 10.1007/s10495-015-1199-9.

 

Book chapters:

Rodrigues J*, Pereira CS*, Fontes N, Gerós H, Côrte-Real M (2018) Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells. In Cláudia Pereira (Eds), Plant Vacuolar Trafficking, vol. 1789, chapter 8. ISBN: 978-1-4939-7855-7. In press *Both authors contributed equally

 

1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), University of Minho, 26-30/9/2016, Braga, Portugal

2nd Joint BIOTECnico and AEM PhD Programs Workshop, Instituto Superior Técnico, 15-16/2/2017, Lisboa, Portugal

2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), University of Minho, 25-29/9/2017, Braga, Portugal

3rd joint  BIOTECnico and AEM PhD programs Workshop, Instituto Superior Técnico10-11/6/2018, Lisboa, Portugal

7rd Workshop of the PhD Program in Applied and Environmental Microbiology (DP_AEM), University of Minho, 24-27/9/2018, Braga, Portugal

3rd Workshop of the PhD Program in Molecular and Environmental Biology (PDBMA), University of Minho, 25/10/2018, Braga, Portugal

8th workshop of the doctoral program in Applied and Environmenral Microbiology (DP_AEM), 10-12/2/2020, Braga, Portugal

Oral Communications:

 

Santos-Pereira C, Andrés MT, Chaves SR, Fierro JF, Gerós H, Manon S, Rodrigues LR, Côrte-Real M (2021) "A genetic and biochemical approach to unveil the mechanisms underlying the antifungal activity of the milk-derived lactoferrin against Saccharomyces cerevisiae", II International Meeting of the Portuguese Society of Genetics, 1st-2nd July 2021 (online).

Santos-Pereira C, Rocha JF, Fernandes HS, Rodrigues LR, Côrte-Real M, Sousa SF (2021) "Deciphering the interaction of lactoferrin with V-ATPase towards a deeper understanding of its mechanisms of action", X Bioinformatics Open Days, 5-7th May, Braga, Portugal (online).

Santos-Pereira C (2021) "Yeast as a tool to elucidate the mechanisms underlying lactoferrin anticancer activity", DP_AEM cycle of online seminars, 14/7, Braga, Portugal (online).

Santos-Pereira C, Guedes JP, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “Lactoferrin is selectively cytotoxic for highly metastatic cancer cells by inhibiting plasmalemmal V-ATPase”, 14th International Conference on Lactoferrin Structure, Function and Applications, 4-8/11, Lima, Peru.

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "How can a milk protein selectively kill cancer cells? Mechanisms underlying lactoferrin-induced apoptosis”, 26th Euroconference on Apoptosis: Cell death in disease: from small molecules to translational medicine, 10-12/9, Saint Petersburg, Russia.

Pereira CS, Guedes JP, Chaves SR, Andrés MT, Fierro JF, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Deciphering the mechanisms underlying bovine milk lactoferrin anticancer activity using yeast and cancer cell lines as complementary models”, XXI Jornadas de Biologia de Leveduras “Professor van Uden, 8-9/6, Braga, Portugal.

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M (2017) "Understanding the interplay between the pro-apoptotic protein lactoferrin and biomembranes: from yeast to human cells". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy.

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Mechanistic insights on the selective anticancer activity of lactoferrin unveiled by yeast". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy.

 

Poster Communications:

Santos-Pereira C, Andrés MT, Rocha JF, Fernandes HS, Chaves SR, Fierro JF, Gerós H, Manon S, Sousa SF, Rodrigues LR, and Côrte-Real, M (2021) Integrated biochemical and computational approach reveals novel mechanisms underlying the antifungal activity of the milk-derived lactoferrin protein. XXI SPB National Congress of Biochemistry. 14-16/10, Évora, Portugal 

Madaleno R, Santos-Pereira C, and Côrte-Real M (2021) Does autophagy play a role in lactoferrin-induced yeast regulated cell death?. II International Meeting of the Portuguese Society of Genetics, 1-2/7, online.

Terra-Matos J, Teixeira MO, Santos-Pereira C, Chaves SR, Sousa MJ, and Côrte-Real M (2021) Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3p display improved ethanol productivity under acetic acid stress. II International Meeting of the Portuguese Society of Genetics, 1-2/7, online.

Santos-Pereira C, Rocha JF, Fernandes HS, Rodrigues LR, Côrte-Real M, Sousa SF (2021) "Elucidating the interaction of lactoferrin with V-ATPase towards understanding its mechanisms of action", AFFINITY 2021 - The 24th meeting of the International Society for Molecular Recognition", 22-24/6, online.

Santos-Pereira C, Rodrigues LR, Côrte-Real M, Cerqueira NMFSA, Sousa SF (2019) “Computational approach to study the interaction between the milk protein lactoferrin and V-ATPase”. Encontro de Jovens Investigadores de Biologia Computacional Estrutural (EJIBCE 2019), 20/12, Lisboa, Portugal. 

Santos-Pereira C, Chaves SR, Andrés MT, Fierro JF, Camougrand N, Manon S, Gerós H, Rodrigues LR and Côrte-Real M (2019) “Which molecular mechanisms are involved in the antifungal activity of the milk-derived protein lactoferrin?”. V symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 10/10, Braga, Portugal.

Teixeira M, Terra-Matos J, Santos-Pereira C, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2019) “The impact of zinc in yeast cell death induced by acetic acid”. V symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 10/10, Braga, Portugal.

Santos-Pereira C, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “The role of membrane lipid domains on lactoferrin anticancer activity: from yeast to human cells”. FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes”, 10-20/6, Cargèse, Corsica, France.

Renduli T, Barbosa A, Pereira CS, Barata C, Mendes F, Pereira H, Ferreira JC, Teixeira M, Silva P, Alves R, Johansson B, Casal M, Sousa MJ and Paiva S. (2019) Yeast as a biotechnological factory. 7º Congresso da Rede de Comunicação de Ciência e Tecnologia de Portugal – SciComPT, 30-31/5, Aveiro, Portugal

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “The role of membrane lipid domains on lactoferrin anticancer activity: from yeast to human cells”. EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function”, 12-16/5, Le Pouliguen, France

Terra-Matos J, Pereira CS, Teixeira M, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2019) “Uncovering the role of vacuolar membrane proteins On Pep4P translocation/vacuolar membrane permeabilization during acetic acid-induced cell death”. I Jornadas da Ciência, 11-15/3, Braga, Portugal

Pereira CS, Rodrigues LR and Côrte-Real M. (2018) “How does the milk protein lactoferrin bind to V-ATPase?”, EMBO course “Computational analysis of protein-protein interactions: Sequences, networks and diseases”, 2018, Sapienza University, Rome, Italy.

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) “Studying the effect of bovine milk lactoferrin on the plasma membrane of yeast and human cells”. IV symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 25/10, Braga, Portugal

Terra-Matos J, Pereira CS, Teixeira M, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2018) “Uncovering the role of vacuolar membrane proteins in acetic acid-induced cell death”. 4th symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 25/10, Braga, Portugal

Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SR and Côrte-Real M (2018) “Yeast Saccharomyces cerevisiae as a model organism to exploit the regulation of the human pro-apoptotic protein Bax”. 4th symposium of PDBMA (Doctoral Program in Applied and Environmental Biology), 25/10, Braga, Portugal

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "How can a milk protein selectively kill cancer cells? Mechanisms underlying lactoferrin-induced apoptosis”, 26th Euroconference on Apoptosis: Cell death in disease: from small molecules to translational medicine, 10-12/9, Saint Petersburg, Russia

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 2nd Small Meeting on Endocytic Trafficking and Signalling (SMETS2), 10-12/7, Braga, Portugal.

Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) " Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

Terra-Matos J, Pereira CS, Sousa MJ, Chaves SC, Gerós H and Côrte-Real M. (2018) " Role of vacuolar membrane proteins in acetic acid-induced cell death ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal 

Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) "Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 1st Meeting on Vesicular Biology, 16-17/2, Coimbra, Portugal.

Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells". Microbiotec 17, 7-9/12, Porto, Portugal. 

Pereira H*, Alves S*, Pereira CS*, Oliveira CFS, Moreira T, Preto A, Chaves SR#Côrte-Real M(2017) "Mitochondrial ROS accumulation sensitizes colorectal cancer cells to acetate and Cathepsin D inhibitors combination therapy". International symposium MitoPorto - Advances in mitochondrial research. 26/5, Porto, Portugal.

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M. (2016) "Insights on the Molecular Mechanisms Underlying the Anticancer Activity of Lactoferrin in Highly Metastatic Cancer Cell Lines". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". 24th  congress of the European Cell Death Organization - "Cell Death in Health and Disease", 28-30/09, Barcelona, Spain.

 

Outreach activities:

Participation on the third Contest on Science Communication in Microbiology promoted by the Portuguese Society of Microbiology with the work entitled “The hidden potential of microbial dark matter”, 25th November 2021.

Organizer and lecturer of the invited workshop “How to perform a scientific poster?” on the National Congress of Medical Students, CNEM2021, 30th October 2021, School of Medicine, Braga, Portugal. 

Event manager of Pint of Science “Com pés e cabeça” and “O nosso mundo”, an international science communication initiative that brings scientists to discuss their latest research with people in a bar, 26th October 2018 to 9th September 2020.

Volunteer under the scope of the project “Voluntariado ECUM” at Oficina São José, a childcare institution from Braga.  This project is promoted by the School of Sciences from UMinho, October 2017 to July 2019.

Collaborator of the “Investigadores voltam à escola” project that brings researchers to schools to talk to kids and teenagers about science and researchers’ daily routines. 18th January 2019, Colégio La Salle, Barcelos, Portugal; 27th March 2019, Colégio Infante D. Henrique, Braga, Portugal.

Organization of the workshop “Sondas Moleculares Fluorescentes” on the meeting “I Jornadas da Ciência”, University of Minho, 14th March 2019

Collaborator of the initiative “UPA - Universidade de Portas Abertas” that aims to communicate science to general society. 4th May 2019.

Member of the organizing committee of the 4th symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 25th October 2018

Volunteer at “European Researchers Night - NEI 2018” in the workshop “Yeast as a cell factory”, 28th September 2018. 

Collaborator of the project “A Minha Escola de Ciências - AMEC” – Workshop: “Citometria de fluxo: uma nova ferramenta para o estudo de microrganismos”, 28th February 2018, Braga, Portugal.

Collaborator of the initiative "De portas abertas à Semana da Ciência e Tecnologia”. Collaboration in the planning and empowerment of the laboratorial activities and explanation of projects and career opportunities, 24th November 2017, Braga, Portugal.

Collaborator of the “Há Ciência na Cidade!” – Workshop: “Perceber o envelhecimento celular com a ajuda do fermento de padeiro”, an initiative of the Biology Department of the School of Sciences from the UMinho directed to general public. 15th July 2017, Braga, Portugal.

Collaborator of the initiative “Open Weekend 2017”, a programme “opened doors” from UMinho directed to students from basic and high school. Collaboration in the planning and empowerment of the laboratorial activities and explanation of projects and career opportunities. 24-25th March 2017, Braga, Portugal.

 

Training

"Metagenomics applied to surveillance of pathogens and antimicrobial resistance" by Technical University of Denmark (DTU) - online through Coursera

 

FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes”, 10-20th June 2019, Cargése, Corsica, France

EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function”, 12-16th May 2019, Le Pouliguen, France

EMBO course: “Computational analysis of protein-protein interactions: Sequences, networks and diseases”, 5-10th Nov 2018, Sapienza University, Rome, Italy

"Protein Degadation and Trafficking in Health and Disease", 2018, University of Minho, Braga

"Mammalian and Yeast cells as models in programmed cell death and autophagy", 2013, University of Minho, Braga

"Cancer therapy: from basic research to clinic", 2013, University of Minho, Braga

"Nanoparticles and the immune system: risks and therapeutics opportunities", 2013, University of Minho, Braga

MIRRI WEBINARS: Microbial resources for a Green, Healthy and Sustainable future & Cutting-edge technologies for 2030 microbial Culture Collections, 18-19th November 2021

 

“Biorender VIZUALIZE 2021 - Advancing communication in science”, virtual event on scientific design, 29-30th September 2021 

 

 

"Biotechnology to build a brighter future: Food, Health and Environmental Applications", online workshop organized by Centre of Biological Engineering, 10th May 2021

 

"Computer simulation and theory of macromolecules", online Hünfeld workshop, organized by Max Planck Institute for Biophysical Chemistry, 23-24th April 2021

 

"Scientific writing - basic principles for a scientific publication", online course organized by Readyto pub, Portugal, 16-18th November 2020

 

"Introduction to Molecular Modelling and Molecular Dynamics in HPC", online course organized by PRACE (Partnership for advanced computing in europe) and GRNET (National Infrastructures for Research and Technology), Greece, 30th June - 1st July 2020

 

"R sessions: introduction to programming languages and R basics, data manipulation, introduction to plotting, writing basic functions and loops, introduction to statistics I and II", University of Minho, Braga, Portugal. Statements of accomplishment of "Introduction to R" and "Intermediate R" emitted by Data Camp, 6th February - 6th March 2020

 

"HADDOCK workshop on Information-driven modelling of biomolecular complexes", University of Coimbra, Coimbra, Portugal 18-19th December 2019

INL summit: Debating the transformation of the global economy, 15-16th October 2018

"Organoids and other advanced tissue models for biomedical research", 20th September 2018, I3S, Porto

“IP Rights in Health & Life Sciences”, 20th September 2018, I3S, Porto

“Fluorescence microscopy”, April 26th 2018, Institute for Science and Innovation in Bio-sustainability (IBS), University of Minho, Braga, Portugal

Cryo Electron Microscopy Workshop: “Enabling Portugal to Become a World Player in Biological Research and Health”, April 20th 2018, International Iberian Nanotechnology Laboratory (INL), Braga, Portugal 

"Wast, spills and personal protective equipment", 15th March, 2017, University of Minho, Braga, Portugal  

 

Communication Skills (course level 1), by Dr. Ana Salgado, 30/9/2016, Braga

Communication Skills (course level 2), by Dr. Ana Salgado, February - July 2017, Braga

Workshop on transferable skills: Self-knowledge, balance of competences, customized and effective CV, preparation for selection interviews. 26/9/2018, by Cátia Marques, University of Minho, Braga

Workshop on transferable skills: customized and effective CV, preparation for selection interviews. 11-12/02/2020, by Cátia Marques, University of Minho, Braga

 

Awards

- Winner of the third Contest on Science Communication in Microbiology promoted by the Portuguese Society of Microbiology with the work entitled “The hidden potential of microbial dark matter”, 25th November 2021. 

 

- Grant for participation in the National Congress of Biochemistry, 14-16th October, 2021

- Best pitch presentation award at the "Microbial Genetics" session of the "II International Meeting of the Portuguese Society of Genetics", 1st-2nd July, 2021

- Young investigator award for poster presentation in the "AFFINITY 2021 - The 24th meeting of the International Society for Molecular Recognition", 22-24th June, 2021

- Best poster presentation award at “Encontro de Jovens Investigadores de Biologia Computacional Estrutural” held in Lisboa, Portugal at 20th December, 2019.

- Student award to the “14th International Conference on Lactoferrin: Structure, Function and Applications” held in Lima, Peru from 4 to 8 November, 2019. 

- FEBS Youth Travel Fund (YTF) to support the participation in the FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes” held in Cargèse, Corsica, France from 10 to 20 June, 2019.

- EMBO travel Grant to attend the EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function” held at Le Pouliguen, France from 12 to 16 May, 2019.

- Scholarship attributed by the European Cell Death Organization (ECDO) to support the attendance to the 26th Conference of the European Cell Death Organization - "Cell death in disease: from small molecules to translational medicine", 10-12th October 2018, Saint Petersburg, Russia.

- “Academic Merit Scholarship” award corresponding to the 2013/2014 school year attributed by Ministério da Ciência, Tecnologia e Ensino Superior at 22th January 2018.

- Best poster presentation award at the symposium “Molecular Microbiology and Microbial Physiology”, MICROBIOTEC 17, 7-9 December 2017, Porto, Portugal

- FEMS Meeting Grant to support the attendance of Young Scientists to the 12th International Meeting on Yeast Apoptosis, 14-18th May, 2017, Bari, Italy.

Current position:

Research Fellow in the scope of the B3IS project - Biodiversity and Bioprospecting of Biosurfactants in Saline Environments (PTDC/BII-BIO/5554/2020). The project aims to discover novel biosurfactants from different saline and hypersaline environments and entails a close partnership between the Centre of Biological Engineering (CEB – University of Minho) and the State Key Laboratory of Lunar and Planetary Science (Macau University of Science and Technology). Work being developed at CEB (March 2021 - present).


Publication

Santos-Pereira C, Andrés MT, Chaves SR, Fierro JF, Gerós H, Manon S, Rodrigues LR, Côrte-Real M (2021) Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae. International Journal of Biological Macromolecules. 171: 343-357. doi: 10.1016/j.ijbiomac.2020.12.224