André
Seixas
...
DP_AEM student
Instituto de Tecnologia Química e Biológica (ITQB)
Doctoral Program in Molecular Biosciences (ITQB)
2018-2022
José Andrade
André
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Phd Thesis

The Interactome of Hfq in the Human Pathogen Listeria monocytogenes

The RNA chaperone Hfq is a conserved RNA-binding protein (RBP) widespread in Gram-negative

and Gram-positive bacteria. Remarkably, inactivation of Hfq causes the strong attenuation of

virulence in the human pathogen Listeria monocytogenes. However, the mechanisms underlying

such phenotype are largely unknown.

The transcriptome and the translatome of Hfq will be analysed, to get the most comprehensive

evaluation of the impact of this RBP in Listeria. Selected candidate genes under control of Hfq will

then be evaluated for their potential to contribute for pathogenicity. This will include small noncoding

RNAs (ncRNAs), as Hfq acts as post-transcriptional regulator of ncRNAs in many bacteria.

However, this function is controversial for Hfq in Listeria. We propose to revisit Hfq association to

ncRNAs and to test alternative functions of Hfq. The strategies defined herein have the potential to

identify new functions for this virulence regulator and to elucidate novel regulatory pathways

governing bacterial virulence.

Publication and Meetings

Dos Santos, R. F., Quendera A.P., Boavida S., Seixas A.F., Arraiano CM., Andrade JM,. Major 3?–5? Exoribonucleases in the Metabolism of Coding and Non-coding RNA. Prog. Mol. Biol. Transl. Sci. 159, 101–155 (2018).

 

Quendera A.P., Seixas A.F., dos Santos, R.F., Santos, I., Silva J.P., Arraiano C.M., Andrade, J.M,. RNA-binding proteins driving the regulatory activity of small non-coding RNAs in bacteria. Frontiers in Molecular Biosciences (in submission).

8th Workshop, February, 2020

Seixas, A.F.; Quendera, A.P.; dos Santos, R.; Silva, J.P.N; Arraiano, C. M. and Andrade, J.M.; Elusive roles of an RNA chaperone in the membrane damage stress response. Microbiotec19, December, Coimbra (poster communication)