Nuno Alexandre
Pedro
...
DP_AEM student
Institute for Bioengineering and Biosciences (IBB)
Doctoral Program in Biotechnology and Biosciences (Técnico)
2018-2021
Nuno Mira
Nuno Alexandre
0
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Phd Thesis

Fostering the development of new anti-Candida therapies based on probiotic lactobacilli

Candida albicans (Ca) and C. glabrata (Cg) are common human commensals but under certain conditions can cause

mucosal infections and, in more serious cases, life-threatening systemic candidiasis. About 75% of women are

estimated to experience vulvovaginal candidiasis during their life-time, a substantial percentage recurrently.

Metagenomics has unveiled an essential role of commensal lactobacilli in vaginal health including in restrain of

candidiasis and in vitro lactobacilli strains were confirmed to inhibit growth and some virulence traits of Candida.

Nonetheless, the mechanisms by which this interference occurs are not known. This PhD program aims to explore this

gap in knowledge to open the door to new intervention therapies aiming to tip the balance in favour of the commensal

lactobacilli by targeting Ca and Cg genes essential for competitiveness and survival in the presence of those bacteria.

In a time of awareness due to the constant emergence of Candida strains resistant to currently used antifungals, the

development of therapeutic approaches based on alternative targets is essential.

Publication and Meetings

2017-2018 - Luísa M. Marques, Marta M. Alves, Sónia Eugénio, Sara B. Salazar, Nuno Pedro, Liliana Grenho, Nuno P. Mira, Maria H. Fernandes, Maria F. Montemor, “Potential anti-cancer and anti-Candida activity of Zn-derived foams” Journal of Materials Chemistry B, 6, 2821-2830, (2018)

2017-2018 - Lourenço, A., Pedro, N., Salazar, S.B., & Mira, N. P. "Effect of acetic acid and lacitc acid at low pH in growth and azole resistance of Candida albicans and Candida glabrata". Frontiers in Microbiology, 9, 3265. (2019)

2018-2019 - Bruna Gonçalves, Ruben Bernardo, Can wang, Nuno A. Pedro, Geraldine Butler, Joana Azeredo, mariana Henriques, Nuno Pereira Mira, Sónia Silva. "Effect of progesterone on Candida Albicans biofilm formation under acidic conditions: a transcriptomic anaysis". International Journal of Medical Microbiology (2019)

7th Workshop of the PhD Program in Applied and Environmental Microbiology (DP_AEM) in University of Minho, Braga from 24-27 September 2018

8th Workshop of the PhD Program in Applied and Environmental Microbiology (DP_AEM) in University of Minho, Braga from 10-13 February 2020

Posters in conferences:

1. Nuno A. Pedro, Marta Alves and Nuno P. Mira. Study of the interaction between vaginal lactobacilli, Candida albicans and Candida glabrata: from physiological aspects to transcriptomic analyses. Poster presented at 14th ASM Conference on Candida and Candidiasis, Providence, USA, 15-19, April (2018).

2. Nuno A Pedro, Catarina Lima, Andreia Pimenta, Sandra C Pinto, Marta Alves, Arsénio Fialho and Nuno P Mira. Study of the interaction between vaginal lactobacilli, Candida albicans and Candida glabrata: from physiological aspects to transcriptomic analyses. Poster presented at HFP2019: Human Fungal Pathogens: Molecular Mechanisms of Host-Pathogen Interactions and Virulence, Nice, France, 18-24, May (2019).

Abstracts in conferences:

1. Nuno A. Pedro and Nuno P. Mira. Study of the interaction between vaginal lactobacilli, Candida albicans and Candida glabrata: from physiological aspects to transcriptomic analyses. Abstract submitted to XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden", Braga, Campus de Gualtar, University of Minho, 8-9, June (2018)

2. Nuno A Pedro, Catarina Lima, Andreia Pimenta, Sandra C Pinto, Marta Alves, Arsénio Fialho and Nuno P Mira. Study of the interaction between vaginal lactobacilli, Candida albicans and Candida glabrata: from physiological aspects to transcriptomic analyses. Abstract submitted to HFP2019: Human Fingal Pathogens: Molecular Mechanisms of Host-Pathogen Interactions and Virulence, Nice, France, 18-24, May (2019).

Oral Presentation in Conferences:

1. Nuno A. Pedro and Nuno P. Mira. Study of the interaction between vaginal lactobacilli, Candida albicans and Candida glabrata: from physiological aspects to transcriptomic analyses. Presented to XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden", Braga, Campus de Gualtar, University of Minho, 8-9, June (2018)

Training

Good computer skills as an office user and other software. Notions of Matlab and easy learning ability of other types of programming or software.