The main challenge to the drinking water (DW) industry is to deliver a product that is microbiologically and chemically safe, aesthetically pleasing and adequate in quantity and delivery pressure. Biofilms constitute one of the major microbial problems in DW distribution systems (DWDS) that most contributes to the deterioration of water quality. However, their elimination from DWDS is almost impossible, but several parameters can be manipulated in order to prevent and control their growth. Fungi are excellent candidates for DW biofilm formation but this aspect is still poorly understood and even less is known about inter-kingdom biofilms involving fungi and bacteria.The purpose of this project is to understand the role of fungi on DW biofilm formation and behaviour. Alterations in the gene expression of the microorganism’s will also be studied as well as the effects of fungi-bacteria interactions in a control context with a DW disinfectant.
Peer-Reviewd International Journals
Costa MS, Rego A, Ramos V, Afonso TB, Freitas S, Preto M, Lopes V, Vasconcelos V, Magalhães C, Leão PN (2016) The conifer biomarkers dehydroabietic and abietic acids are widespread in Cyanobacteria. Scientific Reports 6:23436. DOI: 10.1038/srep23436.
Afonso TB, Costa MS, Rezende de Castro R, Freitas S, Silva A, Schneider MP, Martins R, Leao PN (2016). Bartolosides E-K from a marine coccoid cyanobacterium. Journal of Natural Products. 2016, 79, 2504?2513. DOI: 10.1021/acs.jnatprod.6b00351
§ 1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA); 26–30 September 2016, Campus de Gualtar, University of Minho, Braga, Portugal.
§ 7th Workshop of the Doctoral Program in Applied and Environmental Microbiology; September 24-27, 2018, Campus de Gualtar, University of Minho, Braga, Portugal.
Afonso, T.B., Simões, L.C., Lima, N. Inter-kingdom biofilm formation between bacteria and filamentous fungi isolated from a drinking water distribution system. MicroBiotec17, December 7th-9th, 2017, Porto, Portugal.
CEB Scientific Annual Meeting 2017. University of Minho, Braga, Portugal, th.
Simões, L.C., Afonso, T., Paterson, R. & Lima, N. (2017) The Role of Filamentous Fungi in the Inter-Kingdom Complex Association of Water Biofilms. In: Boosk of Absracts of CEB Annual Meeting 2017 (Ed: E.C. Ferreira) Universidade do Minho, Centro de Engenharia Biológica, p. 27. ISBN: 978-989-97478-8-3. URI: http://hdl.handle.net/1822/46213
Participation in the "Cientista por 1 dia!" activity organized at University of MInho (21st November 2018, CEB-Braga)
Confocal Microscopy Short Course. University of Minho, Braga, Portugal, 22 - 23 May 2017.