Rosana
Alves
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2015-2019
Sandra Paiva
Rosana
0
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Phd Thesis

Function and regulation of Candida albicans and C. glabrata carboxylic acid transporters and their role in biofilm formation, antifungal drug resistance and phagocytosis.

Candida species are the most frequent opportunistic fungal pathogens in humans. Their capacity to cause infection is dependent on the ability to grow within the human host environment and to assimilate the carbon sources available. Previous studies have shown that alternative carbon sources influence the behaviour of these fungal pathogens, suggesting that some carboxylic acid transporters have an important role in biofilm formation and in the acquisition of resistance to antifungal drugs.

In this project, we aim at advancing the knowledge on the function and regulation of carboxylic acids transporters in two important fungal pathogens, C. albicans and C. glabrata, as well as clarifying their role in biofilm formation, antifungal drug resistance and phagocytosis. Considering the different niches in which Candida grows in the body, the results of our project have the potential to fundamentally change our understanding on the interactions between these pathogens and the human host.

 

Publication and Meetings

Oliveira-Pacheco J*,  Alves R*, Costa-Barbosa A, Cerqueira-Rodrigues B, Pereira-Silva, Paiva S, Silva S, Henriques M, Pais C & Sampaio P (2018) The role of Candida albicans transcription fator RLM1 in response to carbon adaptation. Front. Microbiol. *both authors contributed equally

 

Alves R, Mota S, Silva S, Rodrigues CF, Alistair JP, Henriques M, Casal M & Paiva S (2017) The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate. Biofouling. doi: 10.1080/08927014.2017.1392514

 

Mota S, Alves R, Carneiro C, Silva S, Brown AJ, Istel F, Kuchler K, Sampaio P, Casal M, Henriques M & Paiva S (2015) Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. Front. Microbiol. 6:919. doi: 10.3389/fmicb.2015.00919

 

1st Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; Jan 21-22, 2016, Lisbon

1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA); Sep 26–30, 2016, Braga

2nd Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; Feb 15-16, 2017, Lisbon

2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA); Sep 25–29, 2017, Braga

3rd Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; Jun 10-12, 2018, Lisbon

 

 

Alves R, Kastora S, Casal M, Brown AJ, Henriques M & Paiva S. Adapting to survive: how Candida glabrata responds to environmental physiological constrains. 14th ASM Conference on Candida and Candidiasis. April 15-19, 2018; Providence, RI, USA. Abstract selected for presentation.

Gomez A, Miranda A, Alves R, Martins M, Paiva S & De Beule PAA. Simultaneous advanced microscopies for celular dynamics. Imaginenano2018. March 13-15, 2018; Bilbao, Spain. Oral Presentation.

Gonçalves A, Alves R, Casal M, Van Dijck P & Paiva S. Candida glabrata biofilm development on medical devices is modulated by the presence of alternative carbon sources. Microbiotec. December 7-9, 2017; Porto, Portugal. Poster.

Barata-Antunes C, Figueiredo AB, Talaia G, Alves R, Martins V, Gerós H & Paiva S. Study of the human Copper Transporters hCTR1 and hCTR2 using a yeast host. Microbiotec. December 7-9, 2017; Porto, Portugal. Poster.

Alves R, Kastora S, Pinho E, Rodrigues CF, Silva S, Casal M, Brown AJ, Henriques M & Paiva S. Global transcriptome characterization of Candida glabrata biofilms in response to acetate and fluconazole. ImResFun, September 3-7, 2017; Innsbruck, Austria. Poster. 

Alves R, Oliveira-Pacheco J, Cerqueira-Rodrigues B, Paiva S, Silva S, Henriques M & Sampaio P. The impact of carbon source in Candida albicans virulence: participation of RLM1 in host pathogen interaction. XLI Portuguese Genetics Conference, Aveiro, Portugal, June 8-9, 2017. Oral presentation.

Alves R, Oliveira-Pacheco J, Cerqueira-Rodrigues B, Paiva S, Silva S, Henriques M & Sampaio P. Metabolic plasticity as a strategy for virulence: participation of Candida albicans transcription factor RLM1 in host-pathogen interaction. I Symposium of Applied Biochemistry, May 31, 2017; Braga, Portugal. Best Poster Presentation.

Alves R, Dambournet D, Sorkin A, Miranda A, De Beule PAA, Drubin D & Paiva S. Characterization of intracellular trafficking of nutrient transporters using combined fluorescence optical sectioning and nanomechanical mapping atomic force microscopy in mammalian cells. XIX Annual Linz Winter Workshop, February 3-6, 2017; Linz, Austria. Poster.

Alves R, Mota S, Silva S, Rodrigues CF, Brown AJ, Henriques M, Casal M & Paiva S. Lactic acid increases the susceptibility of Candida albicans to fluconazole. XIX National Congress of Biochemistry, Guimarães, Portugal, December 8-10, 2016. Best Oral Communication.

Alves R, Kastora S, Pinho E, Rodrigues CF, Silva S, Casal M, Brown A J, Henriques M & Paiva S. Impact of alternative carbon sources and antifungal treatment on Candida glabrata biofilms’ transcription profile. XIX National Congress of Biochemistry, December 8-10, 2016; Guimarães, Portugal. Poster.

Barata-Antunes C, Talaia G, Alves R, Martins V, Gerós H & Paiva S. Heterologous expression of the Human Copper Transporters hCTR1 and hCTR2 in yeast for endocytic trafficking analysis. XIX National Congress of Biochemistry, December 8-10, 2016; Guimarães, Portugal. Poster.

Alves R, Mota S, Barata-Antunes C, Silva S, Rodrigues CF, Brown A J, Henriques M, Casal M & Paiva S. Carboxylic acid transportes Jen1 and Jen2 affect Candida albicans biofilms’ formation and susceptibility to fluconazole. XL Portuguese Genetics Conference, June 1-2, 2016; Coimbra, Portugal. Poster.

Alves R, Mota S, Carneiro C, Silva S, Brown AJ, Istel F, Kuchler K, Sampaio P, Casal M, Henriques M & Paiva S. Biofilm formation, antifungal drug susceptibility and phagocytosis in Candida glabrata are modulated by the carbon source. 33rd Small Meeting on Yeast and Transport Energetics, Lisbon, Portugal, July 21-24, 2015. Oral Presentation.

Mota S, Alves R, Carneiro C, Silva S, Brown A J, Istel F, Kuchler K, Sampaio P, Casal M, Henriques M & Paiva S. Candida glabrata susceptibility to antifungals and phagocytosis is modulated by the carbon source. XXXIX Portuguese Genetics Conference, Braga, Portugal. May 25-27, 2015. Oral Presentation.

 

 

Volunteer at European Researchers’ Night; September 29, 2017 (Braga Portugal).

Invited speaker at PubhD UMinho, Bar Sé La Vie; March 23, 2017 (Braga, Portugal). 

Invited speaker at DBIO seminars, University of Minho; November 23, 2016 (Braga, Portugal).

Volunteer at QUALIFICA (Educational Trade Fair); Exponor; April 15, 2016 (Porto, Portugal).

Volunteer at Festa da Ciência (Science Festival), May 12-14, 2015 (Braga, Portugal).

 

 

Training

Hands-on Winter School “Advances in Single-Molecule Research for Biology & Nanoscience”, January 31 - February 2, 2017; Linz, Austria.

FEBS Advanced Practical CourseState-of-the-art infection models for human pathogenic fungiFebruary 14-27, 2016; Jena, Germany.,

 

 

Communication Skills (course level 1), 30 September 2016, Braga

Communication Skills (course level 2), from February to July 2017, Braga

Awards

Travel Grant awarded by Luso-American Development Foundation (Portugal, 2018)

Best Poster Presentation at I Symposium of Applied Biochemistry (Portugal, 2017)

Best Oral Presentation at XIX National Congress of Biochemistry (Portugal, 2016)

Travel Grant awarded by European Confederation of Medical Mycology (EU, 2016)

Fulbright Research Grant, awarded by Fulbright Commission (US Department of State, 2015)