Cátia
Pereira
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2016-2020
Manuela Côrte-Real
Cátia
0
0

Phd Thesis

Yeast as a tool to elucidate the anticancer activity of lactoferrin and its bioactive peptide lactoferricin

Publication and Meetings

Peer-Reviewed Articles:

Barbosa C*Santos-Pereira C*, Soares I, Martins V, Terra-Matos J, Côrte-Real M, Lúcio M, Oliveira MECDR, Gerós H (2019) Resveratrol-loaded nanoformulations are internalized by endocytosis in yeast. Journal of Natural Products82, 5, 1240-1249. doi: 10.1021/acs.jnatprod.8b01003 *Both authors contributed equally

 

Costa D, Borges J, Mota MF, Rodrigues MS, Pereira-Silva P, Ferreira A, Pereira CS, Sampaio P, Vaz F (2019) Effect of microstructural changes in the biological behavior of magnetron sputtered ZnO thin films. Journal of Vacuum Science and Technology. 37, 011501. doi.org/10.1116/1.5048785

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M (2018) Bovine Milk Lactoferrin Selectively Kills Highly Metastatic Prostate Cancer PC-3 and Osteosarcoma MG-63 Cells In Vitro. Frontiers in Oncology. doi.org/10.3389/fonc.2018.00200

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394

Acosta-Zaldívar M,Andrés MTRego APereira CSFierro JFCôrte-Real M (2016) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiaeApoptosis. DOI 10.1007/s10495-015-1199-9.

 

Book chapters:

Rodrigues J*, Pereira CS*, Fontes N, Gerós H, Côrte-Real M (2018) Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells. In Cláudia Pereira (Eds), Plant Vacuolar Trafficking, vol. 1789, chapter 8. ISBN: 978-1-4939-7855-7. In press *Both authors contributed equally

 

1st joint workshop of the PhD programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), 26-30/9/2016, Braga, Portugal.

2nd joint BIOTECnico and AEM PhD programs Workshop, 15-16/2/2017, Lisboa, Portugal.

 2nd joint workshop of the PhD programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), 25-29/9/2017, Braga, Portugal.

3rd joint  BIOTECnico and AEM PhD programs Workshop, 10-11/6/2018, Lisboa, Portugal.

7th workshop of the doctoral program in Applied and Environmenral Microbiology (DP_AEM), 24-27/9/2018, Braga, Portugal

8th workshop of the doctoral program in Applied and Environmenral Microbiology (DP_AEM), 10-12/2/2020, Braga, Portugal

Oral Communications:

7. Santos-Pereira C, Guedes JP, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “Lactoferrin is selectively cytotoxic for highly metastatic cancer cells by inhibiting plasmalemmal V-ATPase”, 14th International Conference on Lactoferrin Structure, Function and Applications, 4-8/11, Lima, Peru (selected for flash talk)

6. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "How can a milk protein selectively kill cancer cells? Mechanisms underlying lactoferrin-induced apoptosis”, 26th Euroconference on Apoptosis: Cell death in disease: from small molecules to translational medicine, 10-12/9, Saint Petersburg, Russia (selected for flash talk)

5. Pereira CS, Guedes JP, Chaves SR, Andrés MT, Fierro JF, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Deciphering the mechanisms underlying bovine milk lactoferrin anticancer activity using yeast and cancer cell lines as complementary models”, XXI Jornadas de Biologia de Leveduras “Professor van Uden, 8-9/6, Braga, Portugal

4. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M (2017) "Understanding the interplay between the pro-apoptotic protein lactoferrin and biomembranes: from yeast to human cells". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy (FEMS grant)

3. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Mechanistic insights on the selective anticancer activity of lactoferrin unveiled by yeast". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy

2. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.(Best presentation award)

1. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11thInternational meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal.

 

Poster Communications:

32. Santos-Pereira C, Rodrigues LR, Côrte-Real M, Cerqueira NMFSA, Sousa SF (2019) “Computational approach to study the interaction between the milk protein lactoferrin and V-ATPase”. Encontro de Jovens Investigadores de Biologia Computacional Estrutural (EJIBCE 2019), 20/12, Lisboa, Portugal. (best poster award)

31. Santos-Pereira C, Chaves SR, Andrés MT, Fierro JF, Camougrand N, Manon S, Gerós H, Rodrigues LR and Côrte-Real M (2019) “Which molecular mechanisms are involved in the antifungal activity of the milk-derived protein lactoferrin?”. V symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 10/10, Braga, Portugal.

30. Teixeira M, Terra-Matos J, Santos-Pereira C, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2019) “The impact of zinc in yeast cell death induced by acetic acid”. V symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 10/10, Braga, Portugal.

29. Santos-Pereira C, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “The role of membrane lipid domains on lactoferrin anticancer activity: from yeast to human cells”. FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes”, 10-20/6, Cargèse, Corsica, France.

28. Renduli? T, Barbosa A, Pereira CS, Barata C, Mendes F, Pereira H, Ferreira JC, Teixeira M, Silva P, Alves R, Johansson B, Casal M, Sousa MJ and Paiva S. (2019) Yeast as a biotechnological factory. 7º Congresso da Rede de Comunicação de Ciência e Tecnologia de Portugal – SciComPT, 30-31/5, Aveiro, Portugal

27. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2019) “The role of membrane lipid domains on lactoferrin anticancer activity: from yeast to human cells”. EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function”, 12-16/5, Le Pouliguen, France

26. Terra-Matos J, Pereira CS, Teixeira M, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2019) “Uncovering the role of vacuolar membrane proteins On Pep4P translocation/vacuolar membrane permeabilization during acetic acid-induced cell death”. I Jornadas da Ciência, 11-15/3, Braga, Portugal

25. Pereira CS, Rodrigues LR and Côrte-Real M. (2018) “How does the milk protein lactoferrin bind to V-ATPase?”, EMBO course “Computational analysis of protein-protein interactions: Sequences, networks and diseases”, 2018, Sapienza University, Rome, Italy.

24. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) “Studying the effect of bovine milk lactoferrin on the plasma membrane of yeast and human cells”. IV symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 25/10, Braga, Portugal

23. Terra-Matos J, Pereira CS, Teixeira M, Chaves SR, Sousa MJ, Gerós H and Côrte-Real M (2018) “Uncovering the role of vacuolar membrane proteins in acetic acid-induced cell death”. 4th symposium of the Doctoral Program in Applied and Environmental Biology (PDBMA), 25/10, Braga, Portugal

22. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SR and Côrte-Real M (2018) “Yeast Saccharomyces cerevisiae as a model organism to exploit the regulation of the human pro-apoptotic protein Bax”. 4th symposium of PDBMA (Doctoral Program in Applied and Environmental Biology), 25/10, Braga, Portugal

21. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "How can a milk protein selectively kill cancer cells? Mechanisms underlying lactoferrin-induced apoptosis”, 26th Euroconference on Apoptosis: Cell death in disease: from small molecules to translational medicine, 10-12/9, Saint Petersburg, Russia

20. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 2nd Small Meeting on Endocytic Trafficking and Signalling (SMETS2), 10-12/7, Braga, Portugal.

19. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) " Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

18. Terra-Matos J, Pereira CS, Sousa MJ, Chaves SC, Gerós H and Côrte-Real M. (2018) " Role of vacuolar membrane proteins in acetic acid-induced cell death ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

17. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal (invitation)

16. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) "Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal

15. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 1st Meeting on Vesicular Biology, 16-17/2, Coimbra, Portugal.

14. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells". Microbiotec 17, 7-9/12, Porto, Portugal. (Best Poster Communication award)

13. Pereira H*, Alves S*, Pereira CS*, Oliveira CFS, Moreira T, Preto A, Chaves SR#Côrte-Real M(2017) "Mitochondrial  ROS accumulation sensitizes colorectal cancer cells to acetate and Cathepsin D inhibitors combination therapy". International symposium MitoPorto - Advances in mitochondrial research. 26/5, Porto, Portugal.

12. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

11. Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M. (2016) "Insights on the Molecular Mechanisms Underlying the Anticancer Activity of Lactoferrin in Highly Metastatic Cancer Cell Lines". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

10. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". 24th  congress of the European Cell Death Organization - "Cell Death in Health and Disease", 28-30/09, Barcelona, Spain.

9. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M.(2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". Microbiotec, 10-12/12, Évora, Portugal.

8. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a system model to characterize Bax regulation by individual kinases". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

7. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

6. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11thInternational meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal. 

5. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2014) “Search for mechanisms underlying the anti-tumoral activity of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

4. Gonçalves M, Loureiro L, Pereira CS, Gerós H, Côrte-Real M. (2014) “An in vitroapproach towards the elucidation of the mechanisms of action of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

3. Loureiro L, Gonçalves M, Pereira CS, Côrte-Real M, Gerós H. (2014) “Functional characterization of mitochondrial suspensions by flow cytometry towards the screening of mitochondrial drug targets”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

2. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CSCôrte-Real M, Fierro JF (2014) “Human lactoferrin triggers a caspase- and mitochondrial-dependent programmed cell death in Saccharomyces cerevisiae”. 10th International meeting on yeast apoptosis (IMYA), 14-18/5, Gothenburg, Sweden.

1. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CSCôrte-Real M, Fierro JF (2014) “Mitochondria involvement in human lactoferrin-induced programmed cell death in Saccharomyces cerevisiae”. XX Jornadas de Biologia de Leveduras Professor Nicolau van Uden, 11-12/7, Lisbon, Portugal.

Outreach activities:

- Event manager of Pint of Science “Com pés e cabeça”, an international science communication initiative that brings scientists to discuss their latest research with people in a bar, 26th October 2018 to present.

- Volunteer under the scope of the project “Voluntariado ECUM” at Oficina São José, a childcare institution from Braga.  This project is promoted by the School of Sciences from UMinho, October 2017 to July 2019.

- Collaborator of the “Investigadores voltam à escola” project that brings researchers to schools to talk to kids and teenagers about science and researchers’ daily routines. 18th January 2019, Colégio La Salle, Barcelos, Portugal; 27th March 2019, Colégio Infante D. Henrique, Braga, Portugal.

- Organization of the workshop “Sondas Moleculares Fluorescentes” on the meeting “I Jornadas da Ciência”, University of Minho, 14th March 2019

Collaborator of the initiative “UPA - Universidade de Portas Abertas” that aims to communicate science to general society. 4th May 2019.

- Member of the organizing committee of the 4th symposium of the Doctoral Program in Applied and Environmental Biology(PDBMA), 25th October 2018

- Volunteer at “Noite Europeia dos Investigadores - NEI 2018” in the workshop “Yeast as a cell factory”, 28th September 2018. 

- Collaborator of the project “A Minha Escola de Ciências - AMEC” – Workshop: “Citometria de fluxo: uma nova ferramenta para o estudo de microrganismos”, 28th February 2018, Braga, Portugal.

- Collaborator of the initiative "De portas abertas à Semana da Ciência e Tecnologia”. Collaboration in the planning and empowerment of the laboratorial activities and explanation of projects and career opportunities, 24th November 2017, Braga, Portugal.

- Collaborator of the “Há Ciência na Cidade!” – Workshop: “Perceber o envelhecimento celular com a ajuda do fermento de padeiro”, an initiative of the Biology Department of the School of Sciences from the UMinho directed to general public. 15th July 2017, Braga, Portugal.

- Collaborator of the initiative “Open Weekend 2017”, a programme “opened doors” from UMinho directed to students from basic and high school. Collaboration in the planning and empowerment of the laboratorial activities and explanation of projects and career opportunities. 24-25th March 2017, Braga, Portugal.

Training

FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes”, 10-20th June 2019, Cargése, Corsica, France

EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function”, 12-16th May 2019, Le Pouliguen, France

 

EMBO course: “Computational analysis of protein-protein interactions: Sequences, networks and diseases”, 5-10th Nov 2018, Sapienza University, Rome, Italy

"Protein Degadation and Trafficking in Health and Disease", 2018, University of Minho, Braga

"Mammalian and Yeast cells as models in programmed cell death and autophagy", 2013, University of Minho, Braga

"Cancer therapy: from basic research to clinic", 2013, University of Minho, Braga

"Nanoparticles and the immune system: risks and therapeutics opportunities", 2013, University of Minho, Braga

INL summit: Debating the transformation of the global economy, 15-16th October 2018

"Organoids and other advanced tissue models for biomedical research", 20th September 2018, I3S, Porto

“IP Rights in Health & Life Sciences”, 20th September 2018, I3S, Porto

“Fluorescence microscopy”, April 26th 2018, Institute for Science and Innovation in Bio-sustainability (IBS), University of Minho, Braga, Portugal

Cryo Electron Microscopy Workshop: “Enabling Portugal to Become a World Player in Biological Research and Health”, April 20th 2018, International Iberian Nanotechnology Laboratory (INL), Braga, Portugal

 

"Wast, spills and personal protective equipment", 15th March, 2017, University of Minho, Braga, Portugal 

 

 

Communication Skills (course level 1), by Dr. Ana Salgado, 30/9/2016, Braga

Communication Skills (course level 2), by Dr. Ana Salgado, February - July 2017, Braga

Workshop on transferable skills: Self-knowledge, balance of competences, customized and effective CV, preparation for selection interviews. 26/9/2018, by Cátia Marques, University of Minho, Braga

Workshop on transferable skills: customized and effective CV, preparation for selection interviews. 11-12/02/2020, by Cátia Marques, University of Minho, Braga

 

Awards

- Best poster presentation award at “Encontro de Jovens Investigadores de Biologia Computacional Estrutural” held in Lisboa, Portugal at 20th December, 2019.

- Student award to the “14th International Conference on Lactoferrin: Structure, Function and Applications” held in Lima, Peru from 4 to 8 November, 2019. 

- FEBS Youth Travel Fund (YTF) to support the participation in the FEBS-EMBO lecture course “Molecular Organization, Function and Dynamics of Biomembranes” held in Cargèse, Corsica, France from 10 to 20 June, 2019.

- EMBO travel Grant to attend the EMBO course “Caveolae and nanodomains: Translating structural principles and dynamics into function” held at Le Pouliguen, France from 12 to 16 May, 2019.

- Scholarship attributed by the European Cell Death Organization (ECDO) to support the attendance to the 26th Conference of the European Cell Death Organization - "Cell death in disease: from small molecules to translational medicine", 10-12th October 2018, Saint Petersburg, Russia.

- “Academic Merit Scholarship” award corresponding to the 2013/2014 school year attributed by Ministério da Ciência, Tecnologia e Ensino Superior at 22th January 2018.

- Best poster presentation award at the symposium “Molecular Microbiology and Microbial Physiology”, MICROBIOTEC 17, 7-9 December 2017, Porto, Portugal

- FEMS Meeting Grant to support the attendance of Young Scientists to the 12th International Meeting on Yeast Apoptosis, 14-18th May, 2017, Bari, Italy.


Publication

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394