Cátia
Pereira
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2016-2020
Manuela Côrte-Real
Cátia
0
0

Phd Thesis

Yeast as a tool to elucidate the anticancer activity of lactoferrin and its bioactive peptide lactoferricin

Publication and Meetings

Peer-Reviewed Articles:

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M (2018) Bovine Milk Lactoferrin Selectively Kills Highly Metastatic Prostate Cancer PC-3 and Osteosarcoma MG-63 Cells In Vitro. Frontiers in Oncology. doi.org/10.3389/fonc.2018.00200

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394

Acosta-Zaldívar M, Andrés MTRego APereira CSFierro JFCôrte-Real M (2016) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiaeApoptosis. DOI 10.1007/s10495-015-1199-9.

 

Book chapters:

Rodrigues J*, Pereira CS*, Fontes N, Gerós H, Côrte-Real M (2018) Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells. In Cláudia Pereira (Eds), Plant Vacuolar Trafficking, vol. 1789, chapter 8. ISBN: 978-1-4939-7855-7. In press *Both authors contributed equally

 

1st joint workshop of the PhD programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), 26-30/9/2016, Braga, Portugal.

2nd joint BIOTECnico and AEM PhD programs Workshop, 15-16/2/2017, Lisboa, Portugal.

 

2st joint workshop of the PhD programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), 25-29/9/2017, Braga, Portugal.

 

Oral Communications:

4. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M (2017) "Understanding the interplay between the pro-apoptotic protein lactoferrin and biomembranes: from yeast to human cells". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy (FEMS grant)

3. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Mechanistic insights on the selective anticancer activity of lactoferrin unveiled by yeast". 12th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy

2. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal. (Best presentation award)

1. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11thInternational meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal.

 

Poster Communications:

17. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal (invitation)

16. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) "Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal

15. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 1st Meeting on Vesicular Biology, 16-17/2, Coimbra, Portugal.

14. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells". Microbiotec 17, 7-9/12, Porto, Portugal. (Best Poster Communication award)

13. Pereira H*, Alves S*, Pereira CS*, Oliveira CFS, Moreira T, Preto A, Chaves SR#Côrte-Real M(2017) "Mitochondrial  ROS accumulation sensitizes colorectal cancer cells to acetate and Cathepsin D inhibitors combination therapy". International symposium MitoPorto - Advances in mitochondrial research. 26/5, Porto, Portugal.

12. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

11. Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M. (2016) "Insights on the Molecular Mechanisms Underlying the Anticancer Activity of Lactoferrin in Highly Metastatic Cancer Cell Lines". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

10. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". 24th  congress of the European Cell Death Organization - "Cell Death in Health and Disease", 28-30/09, Barcelona, Spain.

9. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M.(2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". Microbiotec, 10-12/12, Évora, Portugal. 

8. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a system model to characterize Bax regulation by individual kinases". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

7. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

6. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11thInternational meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal. 

5. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2014) “Search for mechanisms underlying the anti-tumoral activity of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

4. Gonçalves M, Loureiro L, Pereira CS, Gerós H, Côrte-Real M. (2014) “An in vitroapproach towards the elucidation of the mechanisms of action of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

3. Loureiro L, Gonçalves M, Pereira CS, Côrte-Real M, Gerós H. (2014) “Functional characterization of mitochondrial suspensions by flow cytometry towards the screening of mitochondrial drug targets”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

2. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CSCôrte-Real M, Fierro JF (2014) “Human lactoferrin triggers a caspase- and mitochondrial-dependent programmed cell death in Saccharomyces cerevisiae”. 10th International meeting on yeast apoptosis (IMYA), 14-18/5, Gothenburg, Sweden.

1. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CSCôrte-Real M, Fierro JF (2014) “Mitochondria involvement in human lactoferrin-induced programmed cell death in Saccharomyces cerevisiae”. XX Jornadas de Biologia de Leveduras Professor Nicolau van Uden, 11-12/7, Lisbon, Portugal.

Oral communication:

Pereira CS (2018) "How can a milk protein selectively kill cancer cells?". DBio seminairs, 7/2, Braga, Portugal (invited speaker)

 

Workshops:

Workshop "Wast, spills and personal protective equipment", 15th March, 2017, Braga, Portugal

 Cryo Electron Microscopy Workshop: “Enabling Portugal to Become a World Player in Biological Research and Health”, April 20th 2018, International Iberian Nanotechnology Laboratory (INL), Braga, Portugal 

 

 Outreach activities:

 Volunteer under the scope of the project “Voluntariado ECUM” since 17 October 2017 until present at Oficina São José, a childcare institution from Braga.  This project is promoted by the School of Sciences from UMinho.

Collaborator of the project “A Minha Escola de Ciências - AMEC” – Workshop: “Citometria de fluxo: uma nova ferramenta para o estudo de microrganismos”, 28th February 2018, Braga, Portugal.

 Collaborator of the initiative "De portas abertas à Semana da Ciência e Tecnologia”. Collaboration in the planning and empowerment of the laboratorial activities and explanation of projects and career opportunities. 24th November 2017, Braga, Portugal.

Collaborator of the “Há Ciência na Cidade!” – Workshop: “Perceber o envelhecimento celular com a ajuda do fermento de padeiro”. 15th July 2017, Braga, Portugal.

Supervisor of the ”Ciência Viva” internship: “Como é que a levedura pode desvendar os segredos de processos envolvidos na morte celular”. 17-21th July 2017, Braga, Portugal. Co-supervised with Manuela Côrte-Real and Joana Matos.

Supervisor of the bachelors in Applied Biology internship, Catarina Moreira Ribeiro, June, 2017. Co-supervised with Manuela Côrte-Real.

Supervisor of the ”Ciência Viva” internship: “Da levedura a novas terapias para novas patologias humanas – “os pequenos” podem ter grandes missões!”. Mariana Ferreira. 25-29th June 2016, Braga, Portugal. Co-supervised with Manuela Côrte-Real and Lisandra Castro.

Supervisor of the ”Ciência Viva” internship: “Da levedura a novas terapias para novas patologias humanas – “os pequenos” podem ter grandes missões!”. Carolina Carmo. 25-29th June 2016, Braga, Portugal. Co-supervised with Manuela Côrte-Real and Lisandra Castro.

Training

"Mammalian and Yeast cells as models in programmed cell death and autophagy", 2013, University of Minho, Braga

"Cancer therapy: from basic research to clinic", 2013, University of Minho, Braga

"Nanoparticles and the immune system: risks and therapeutics opportunities", 2013, University of Minho, Braga

 

 

Communication Skills (course level 1), by Dr. Ana Salgado, 30 September 2016, Braga

Communication Skills (course level 2), by Dr. Ana Salgado, February - July 2017, Braga

Awards

 “Academic Merit Scholarship” award corresponding to the 2013/2014 school year attributed by Ministério da Ciência, Tecnologia e Ensino Superior at 22th January 2018.

 Best poster presentation award at the symposium “Molecular Microbiology and Microbial Physiology”, MICROBIOTEC 17, 7-9 Dez 2017, Porto, Portugal

FEMS Meeting Grant to support the attendance of Young Scientists to the 12th International Meeting on Yeast Apoptosis, 14-18th May, 2017, Bari, Italy.

Best presentation award on the "III Symposium of the PhD Programme on Molecular and Environmental Biology", 26th May, 2015 Braga, Portugal


Publication

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394