Ana Luísa
Pereira
...
DP_AEM student
Centro de Engenharia Biológica (CEB)
Doctoral Program in Chemical and Biological Engineering (CEB)
2016-2020
Madalena Alves
Ana Luísa
0
0

Phd Thesis

Syngas fermentation using pressurized systems

A main focus of current environmental research is the study and development of new technologies to produce renewable bio-based fuels and chemicals. In what concerns the biological processes, the fermentation of lignocellulosic materials has received a great deal of attention, but limitations on the hydrolysis and low biodegradability of lignin are still hampering the implementation of this process. Gasification of biomass and further bioconversion of the synthesis gas (syngas) appears to be a promising alternative to the traditional fermentation process. Gasification allows almost complete conversion of carbonaceous materials to a syngas mixture, containing mainly CO, CO2 and H2. Additionally, the biocatalysts (microorganisms) used for syngas conversion are more resistant to impurities present in bio-syngas than the traditional metal catalysts. However, the biggest challenge in syngas bioconversion is to overcome the gas-liquid mass transfer limitations. Those limitations are related to the struggle to transfer the low-solubility gas components to the aqueous phase (where the biological reactions take place). Thus, the development of systems that promote the enhancement of mass transfer coefficient will increase productivity rates. Besides, a deeper study of the biocatalysts involved in syngas bioconversion and the respective pathways, will also contribute to boost the application of this technology.

In this project, it is proposed to improve syngas bioconversion by increasing gas-liquid mass transfer and consequently product yields, and directing the process to produce compounds with high economical and industrial value.

The project foresees the use of different pressurized bio-reactors designs (gas-lift bioreactor and axial agitation bioreactor), with syngas pressures up to 20 bar, with the goal to reduce gas-liquid mass transfer limitations by increasing syngas components solubility.

Publication and Meetings

·         Arantes A. L., Alves J. I., Stams A. J. M., Alves M. M., Sousa D. Z. (2016) Enrichment of syngas-converting communities from a multi-orifice baffled bioreactor, Microbial biotechnology 0(0), 000–000 (2017) DOI: 10.1111/1751-7915.12864

·         Lopes M., Alves J. I., Arantes A.L., Belo I., Sousa D.Z., Alves M.M.. (2015) Hydrogenotrophic activity under increased H2/CO2 pressure: effect on methane production and microbial community. J Biotechnol 208(Suppl.): S57.

·         1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 26-30 September 2016

·         2nd Joint BIOTECnico and AEM PhD Programs Workshop, Instituto Superior Técnico, Lisboa, Portugal, 15-16 February 2017

·       2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 25-29 September 2017

·         Arantes A. L., Alves J. I., Stams A. J. M., Alves M. M., Sousa D. Z., Microbial diversity of anaerobic syngas-converting enrichments from a multi-orifice baffled bioreactor (MOBB). ICBM-3 - 3rd International Conference on Biogas Microbiology. Wageningen, The Netherlands, 1-3 May 2017. (oral communication)

·         Alves J.I., Lopes M., Arantes A.L., Sousa D.Z., Alves M.M. Pressurized syngas bioconversion: physiological and microbial characterization. 3rd International Conference on Biogas Microbiology (ICBM-3), May 1-3, Wageningen, The Netherlands, 2017. (oral communication)

·         Arantes A. L., Alves J. I., Lopes M., Sousa D.Z., Belo I., Alves M.M., Pressurized syngas bioconversion: turning gaseous residues into valuable products. 2017 International Workshop on Environment and Alternative Energy, Noordwijk, The Netherlands, 30 May - 2 June 2017. (oral communication)

·         Alves J.I., Arantes A.L., Lopes M., Belo I., Sousa D.Z., Alves M.M. Perspectives on syngas fermentation. CEB Annual Meeting 2017, July 6, Braga, Portugal, 2017. ISBN: 978-989-97478-8-3 (oral communication)

·         Arantes A. L., Alves J. I., Stams A. J. M., Alves M. M., Sousa D. Z., Microbial diversity of anaerobic syngas-converting enrichments from a multi-orifice baffled bioreactor (MOBB). MICROBIOTEC17, December 7-9, Porto, Portugal, 2017.(poster)

·         Alves J.I., Lopes M., Arantes A.L., Belo I., Sousa D.Z., Alves M.M. Pressurized syngas bioconversion: physiological and microbial characterization. MICROBIOTEC17, December 7-9, Porto, Portugal, 2017.  (poster)

 

·         Symposium Novel Anaerobes 2017, University of Minho, Braga, Portugal, 10th November 2017

Training

“Hands-on course: Introduction to biological data analysis with R”, at i3S – Instituto de Investigação e Inovação em Saúde, Porto, 26-28 June 2017 (18 hours).

- Matlab and SuperPro Designer Workshop, at CEB – Centre of Biological Engineering, Braga, 29-30 June 2017 (14 hours).

Soft skills course with Dr. Ana Salgado, Braga

- Soft skills course project (on going)

- Seminar “How to design and defend your thesis with the Idea Puzzle software” held in University of Minho, Braga, Portugal, 19 March 2018.

 


Publication

? Arantes, A.L., Alves, J.I., Stams, A.J.M., Alves M.M. and Sousa D.Z. (2016) Microbial diversity of syngas degrading enrichments from a multi-orifice baffled bioreactor (MOBB). (Manuscript in preparation)

? Lopes M., Alves, J.I., Arantes, A.L., Belo, I., Sousa, D.Z., Alves, M.M. (2015) Hydrogenotrophic activity under increased H2/CO2 pressure: effect on methane production and microbial community. J Biotechnol 208(Suppl.): S57.

? Marlene Lopes, Joana Isabel Alves, Ana Luísa Arantes, Isabel Belo, Diana Zita Sousa, M. Madalena Alves, “Hydrogenotrophic activity under increased H2/CO2 pressure: effect on methane production and microbial community”. Proceedings of EUROPEAN BIOTECHNOLOGY CONGRESS, 7th – 9th May, Bucharest, Romania, 2015. (Poster presentation)

? Ana Luísa Arantes, Alfons J. M. Stams, M. Madalena Alves, Diana Zita SousaEnrichment of carbon monoxide utilising microorganisms from methanogenic bioreactor sludge”. Proceedings of SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 27th – 30th April, La Jolla, San Diego, USA, 2015. (Oral presentation)

? Ana Luísa Arantes, Alfons J. M. Stams, M. Madalena Alves, Diana Zita Sousa,Enrichment of carbon monoxide utilising microorganisms from methanogenic bioreactor sludge”. Proceedings of XI LATINAMERICAN SYMPOSIUM OF ANAEROBIC DIGESTION, 24th-27th November, La Havana, Cuba, 2014. (Poster presentation)