Ana Margarida
Pereira
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2015-2019
Raul Machado
Ana Margarida
0
0

Phd Thesis

Insights into the mechanism of antimicrobial activity and its exploitation for developing new therapies against clinically relevant bacteria

Antimicrobial resistance is a serious worldwide threat to public health that is emerging due to de misuse and overuse of antimicrobials. In fact, infectious diseases are the second most frequent cause of death worldwide. Thus, it is of foremost importance to discover new therapeutic approaches against microbial pathogens. Antimicrobial peptides (AMPs) are promising alternatives to classical antimicrobials, especially because the acquisition of resistance to AMPs is significantly reduced. In general, extraction of these peptides from natural sources or by chemical synthesis is a cumbersome, complex and expensive process that does not provide an efficient method to obtain peptides in large amounts. One way to potentially overcome these drawbacks is by exploring the use of recombinant DNA technology for the biotechnological production of AMPs using microbial cell factories. Nevertheless, due to their nature, AMPs are usually expressed in insoluble inclusion bodies and are susceptible to proteolysis. The use of recombinant protein-based polymers (rPBPs) that act as polymer fusion partners presents several advantages: increases the solubility of the AMPs; function as purification tag and allows the creation of functionalized antimicrobial biomaterials. Owing to the unique balance between their mechanical properties, biocompatibility, biodegradability and thermostability, elastin-like recombinamers (ELRs) and silk-elastin like proteins (SELPs) are two of the most remarkable families of rPBPs for biotechnological applications, particularly for the biomedical field.

Publication and Meetings

PEER - REVIEWED INTERNATIONAL ARTICLES:

da Costa,A., Pereira A.M., Gomes, A.C., Rodriguez-Cabello, J.C., Sencadas, V., Casal, M., Machado, R. 2018. Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer. New Biotechnology,46, 45-53.

da Costa,A., Pereira A.M., Gomes, A.C., Rodriguez-Cabello, J.C., Sencadas, V., Casal, M., Machado, R. 2017. Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer. Biomedical Materials, 12(4),045011.

A. M. Pereira, R. Machado, A. da Costa, A. Ribeiro, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal. 2017. Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47, 50-59.

Machado R., da Costa A., Sencadas V., Pereira A. M., Collins T., Rodríguez-Cabello J.C., Lanceros-Méndez S., Casal M. 2015. Exploring the properties of genetically engineered Silk-Elastin Like Protein Films. Macromolecular Bioscience, 15(12), 1698-1709.

Ferreira, C., Pereira A. M., Pereira, M. C., Simões, M. and Melo, L.F. 2013. Biofilm control with new microparticles with immobilized biocide. Heat Transfer Engineering, 34, 712-718.

Pereira A. M., Abreu, A. C., M., Simões M., Action of kanamicyn against single and dual species biofilms of Escherichia coli and Staphylococcus aureus. Journal of Microbiology Research, 2, 84-88.

Simões, L. C., Lemos, M., Pereira, A.M., Abreu C, Saavedra M J, Simões, M. 2011. Persister cells in a biofilm treated with a biocide. Biofouling 27, 403-411.

Simões, L. C., Lemos, M., Araújo, P., Pereira, A.M., Simões, M. 2011. The effects of glutaraldehyde on the control of single and dual biofilms of Bacillus cereus and Pseudomonas fluorescens. Biofouling 27, 337-346.

Ferreira C., Pereira A.M., Pereira M.C., Melo LF and Simões M. 2011. Physiological changes induced by the quaternary ammonium compound benzyldimethyldodecylammonium chloride on Pseudomonas fluorescens. Journal of Antimicrobial Chemotherapy 66, 1036-1043.

 

INTERNATIONAL BOOK CHAPTERS:

Ferreira C., Pereira A. M., Melo L.F., Simões M., “ADVANCES IN INDUSTRIAL BIOFILM CONTROL WITH MICRO-NANOTECHNOLOGY” In: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex, 2010

·       1st Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; January 21-22, 2016, Lisboa

·     1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA); 26–29/30 September 2016, Braga

·       2nd Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; February 15-16, 2017, Lisboa

·      2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA); 25–29 September 2017, Braga

·       3rd Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP; June 11-12, 2018, Lisboa.

7th WORKSHOP OF THE DOCTORAL PROGRAM IN APPLIED AND ENVIRONMENTAL MICROBIOLOGY; September 24-27, 2018, Braga

A. M. Pereira, A. da Costa, S. Campos-Dias, I. Sá-Correia, M. Casal, R. Machado. Biotechnological production of antimicrobial peptides using protein polumer tags, presented at the  XLII Portuguese Genetics Conference, June 14th-15th, 2018, Porto, Portugal

A. M. Pereira, A. da Costa, S. Campos-Dias, I. Sá-Correia, M. Casal, R. Machado. Development of antimicrobial protein-based polymers for biomedical applications, presented at the 19th Biennial Meeting for the Society for Free Radical Research International (SFRRI)/ XX Congresso Nacional de Bioquímica, June 4th-7th 2018, Lisbon, Portugal.

A. M. Pereira, A. da Costa, I. Sá-Correia, M. Casal, R. Machado. Developing antimicrobial materials for biotechnological applications, presented at MicroBiotec17, December 7th-9th, 2017, Porto, Portugal

A. M. Pereira, A. da Costa, S. Campos-Dias, I. Sá-Correia, M. Casal, R. Machado. Fabrication of polymeric protein-based materials with antimicrobial propertiesfor biomedical applications, presented at the International Symposium on Bioinspired Macromolecular Systems (ISBMS), November 6th-8th 2017, Aveiro, Portugal 

A. M. Pereira, A. da Costa, I. Sá-Correia, M. Casal, R. Machado. Genetically engineered antimicrobial biopolymers as promising therapeutics for biomedical application. XLI Portuguese Genetics Conference, June 8th-9rd, 2017, Aveiro, Portugal

Pereira, A. M., Machado, R., da Costa, A., Bernardo, T.C., Ribeiro, A., Collins, T., Gomes, A. C., Leonor, I. B., Kaplan, D., Reis, R. L., Casal, M. Silk-based biomaterials with improved cell adhesion properties, XIX National Congress of Biochemestry, December 8th-10th, 2016, Guimarães, Portugal

A. M. Pereira, R. Machado, A. da Costa, A. Ribeiro, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal, Genetically engineered silk-based composite biomaterials functionalized with fibronectin type-II that promote cell adhesion, presented at XL Portuguese Genetics Conference, June 2nd-13rd, 2016, Coimbra, Portugal

A. M. Pereira, R. Machado, T.C. Bernardo, A. da Costa, A. Ribeiro, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal, Advanced silk-based genetic polymers with improved cell adhesion properties, presented at MicroBiotec15, December 10th-12th, 2015, Évora, Portugal

A. M. Pereira, R. Machado, T. C. Bernardo, A. da Costa, A. Ribeiro, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal, “Silk-based polymers functionalized with fibronectin type-II for improved cell adhesion” presented at the XXXIX Portuguese Genetics Conference, May 25th-27th, 2015, Braga, Portugal

A.M. Pereira, T.C. Bernardo, A. da Costa, T. Collins, R. Machado, I.B. Leonor, M. Casal, R.L. Reis, “Development of new chimeric proteins for tissue engineering”, presented in Term Stem 2013, October 10th-12th,2013, Porto, Portugal

A.M. Pereira, T.C. Bernardo, A. da Costa, T. Collins, R. Machado, I.B. Leonor, M. Casal, R.L. Reis, “Development of new chimeric proteins for tissue engineering”, presented in the 1st Workshop of Polaris, October 7th-9th, 2013, Porto, Portugal

A.M. Pereira, T.C. Bernardo, A. da Costa, T. Collins, R. Machado, I.B. Leonor, M. Casal, R.L. Reis, “Development of new chimeric proteins for tissue engineering”, presented in the 3rd ICVS/3B's Meeting, June 28th, 2013, Braga, Portugal

Maria J. Feio, Peter Eaton, A. Margarida Pereira, Paula Gameiro, Inês Gomes and Ricardo Franco, “Cytochrome c interactions with gold surfaces and nanoparticles”, presented in the 4th Requimte Meeting, March 31st/April 1st, 2006, Fátima, Portugal.

 

       GlycoBiology Summer School (September 23-25, 2015, Braga)

       Participation in the photography contest "Science in the Transformation of the World" promoted by the School of Sciences of the University of Minho in the academic year 2016/2017. 

       Participation at “Semana da Ciência e Tecnologia” organized at University of Minho (24 Nov 2017, CBMA-Braga).

       Participation in the world science communication competition Fame Lab: selected for the regional elimination at Braga, 6 February.

       Participation at DBIO Seminars: "Antimicrobial peptides: powerful allies to tackle antimicrobial resistance" (CBMA, Braga, 18 April 2018)

      Participation at PubhD UMinho, June 21st, Café Convcerto of Centro Cultural de Vila Flor, Guimarães.

Training

"Biopolymers based on renewable resources: from synthesis to applications” (April 11- April 22, 2016)

"Nanoparticles: Challenges and therapeutic opportunities" (May 23-June 3, 2016)

"Bionanosystems for pharmaceutical and cosmetic applications” (June 20 – July 1, 2016)

 

·       Communication Skills (course level 1), 30 September 2016, Braga

·       Communication Skills (course level 2), from February to July 2017, Braga

Awards

·       Awarded with grant of the Portuguese Society in Biochemistry to attend the XIX National Congress of Biochemistry.

·       Awarded the 2nd Prize in the Photography Contest "Science in the Transformation of the World" promoted by the School of Sciences of the University of Minho in the academic year 2016/2017.


Publication

A. M. Pereira, R. Machado, A. da Costa, A. Ribeiro, T. Bernardo, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal. 2017. Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47, 50-59.