Ana Margarida
Pereira
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2015-2019
Raul Machado
Ana Margarida
0
0

Phd Thesis

Insights into the mechanism of antimicrobial activity and its exploitation for developing new therapies against clinically relevant bacteria

Antimicrobial resistance is a serious worldwide threat to public health that is emerging due to de misuse and overuse of antimicrobials. In fact, infectious diseases are the second most frequent cause of death worldwide. Thus, it is of foremost importance to discover new therapeutic approaches against microbial pathogens. Antimicrobial peptides (AMPs) are promising alternatives to classical antimicrobials, especially because the acquisition of resistance to AMPs is significantly reduced. In general, extraction of these peptides from natural sources or by chemical synthesis is a cumbersome, complex and expensive process that does not provide an efficient method to obtain peptides in large amounts. One way to potentially overcome these drawbacks is by exploring the use of recombinant DNA technology for the biotechnological production of AMPs using microbial cell factories. Nevertheless, due to their nature, AMPs are usually expressed in insoluble inclusion bodies and are susceptible to proteolysis. The use of recombinant protein-based polymers (rPBPs) that act as polymer fusion partners presents several advantages: increases the solubility of the AMPs; function as purification tag and allows the creation of functionalized antimicrobial biomaterials. Owing to the unique balance between their mechanical properties, biocompatibility, biodegradability and thermostability, elastin-like recombinamers (ELRs) and silk-elastin like proteins (SELPs) are two of the most remarkable families of rPBPs for biotechnological applications, particularly for the biomedical field.

Publication and Meetings

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Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.

Training

Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.

 

Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.

Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.

Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.

Awards

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Lorem ipsum dolor sit amet, quo ex detracto pericula, modo munere commodo his et. Natum salutatus dissentiet pri et, nisl utamur eu ius, et ancillae electram sit. Offendit lucilius disputando ut his, altera deserunt temporibus eum ea, mel ex modo epicuri. Vim ea habeo evertitur, deserunt patrioque sit ad, et dicam adipiscing cum. Ad eam nibh posse, appetere percipit ne duo, fabulas facilisis cum ut.


Publication

A. M. Pereira, R. Machado, A. da Costa, A. Ribeiro, T. Bernardo, T. Collins, A. C. Gomes, I. B. Leonor, D. Kaplan, R. L. Reis, M. Casal. 2017. Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47, 50-59.

Machado, R., da Costa, A., Sencadas, V., Pereira, A. M., Rodríguez-Cabello, J. C., Lanceros-Mendez, S., Casal, M. 2015. Exploring the properties of genetically engineered silk-elastin-like protein films. Macromolecular Bioscience 15(12), 1698-709

Ferreira C., Pereira A.M., Pereira M.C., Melo LF and Simões M. 2011. Physiological changes induced by the quaternary ammonium compound benzyldimethyldodecylammonium chloride on Pseudomonas fluorescens. Journal of Antimicrobial Chemotherapy 66, 1036-1043.

Simões, L. C., Lemos, M., Araújo, P., Pereira, A.M., Simões, M. 2011. The effects of glutaraldehyde on the control of single and dual biofilms of Bacillus cereus and Pseudomonas fluorescensBiofouling 27, 337-346.

Simões, L. C., Lemos, M., Pereira, A.M., Abreu C, Saavedra M J, Simões, M. 2011. Persister cells in a biofilm treated with a biocide. Biofouling 27, 403-411.

Pereira A. M., Abreu, A. C., M., Simões M., Action of kanamicyn against single and dual species biofilms of Escherichia coli and Staphylococcus aureus. Journal of Microbiology Research, 2, 84-88.

Ferreira, C., Pereira A. M., Pereira, M. C., Simões, M. and Melo, L.F. 2013. Biofilm control with new microparticles with immobilized biocide. Heat Transfer Engineering, 34, 712-718.