Maria Antónia
Silva
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2016-2020
Isabel Soares Silva
Maria Antónia
0
0

Phd Thesis

Identification and functional characterization of novel plasma membrane carboxylate transporters

Organic acids such as monocarboxylic acids, dicarboxylic acids or even more complex molecules such as sugar acids, have displayed great applicability in the industry as these compounds are used as platform chemicals for polymer, food, agricultural and pharmaceutical sectors. Chemical synthesis of these compounds from petroleum derivatives is currently their major source of production. However, increasing environmental concerns have prompted the production of organic acids by microorganisms. The current trend is the exploitation of industrial biowastes to sustain microbial cell growth and valorize biomass conversion into organic acids. One of the major bottlenecks for the efficient and cost-effective bioproduction is the export of organic acids through the microbial plasma membrane. Membrane transporter proteins are crucial elements for the optimization of substrate import and final product export. Several transporters have been expressed in organic acid-producing species, resulting in increased final product titers in the extracellular medium and higher productivity levels. In this project, we aim to identify new carboxylate transporters and explore its possible biotechnological applications for CA production.

Publication and Meetings

Peer-Reviewed Articles:

Soares-Silva, I., Ribas, D., Sousa-Silva, M., Azevedo-Silva, J., Rendulic, T., & Casal, M. (2020). Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications. FEMS Microbiology Letters, 367(15), fnaa118.

Alves, R., Sousa-Silva, M., Vieira, D., Soares, P., Chebaro, Y., Lorenz, M.C., Casal, M., Soares-Silva, I. & Paiva, S. (2020) Carboxylic Acid Transporters in Candida Pathogenesis, Mbio. 11(3), 1-11.

Ribas, D., Soares-Silva, I., Vieira, D., Sousa-Silva, M., Sá-Pessoa, J., Azevedo-Silva, J., Viegas, S., Arraiano, C., Diallinas, G., Paiva, S., Soares, P. & Casal, M. (2019) The acetate uptake transporter family motif “NPAPLGL (M/S)” is essential for substrate uptake, Fungal Genetics and Biology. 122, 1-10.

Sousa-Silva, M., Simões, M., Melo, L. & Machado, I. (2018Pseudomonas fluorescens tolerance to benzyldimethyldodecyl ammonium chloride: altered phenotype and cross-resistance. Journal of Global Antimicrobial Resistance. 15, 188-195. DOI: 10.1016/j.jgar.2018.07.004

 

ORCID: 0000-0003-1622-4888

Linkedin: linkedin.com/in/maria-antónia-silva-64847563

 

1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 26-30 Sept 2016

2nd Joint BIOTECnico and AEM PhD Programs Workshop, Instituto Superior Técnico, Lisbon, Portugal, 15-16 Feb 2017

2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 25-29 Sep 2017

3rd Joint BIOTECnico and AEM PhD PROGRAMS WORKSHOP in IST, Lisbon from 11-12 June 2018

7rd Workshop of the PhD Program in Applied and Environmental Microbiology (DP_AEM) and Molecular in University of Minho, Braga from 24-27 September 2018

3rd Workshop of the PhD Program in Molecular and Environmental Biology (PDBMA) in University of Minho, Braga at 25 October 2018

 

 

Oral Communications:

 10. Maria Sousa-Silva, Daniel Vieira, David Ribas, Pedro Soares, Margarida Casal, Isabel Soares-Silva, “Exploring the Cyberlindnera jadinii yeast transportome to uncover novel carboxylate transporters for biotechnological applications”, Oral Communication in Molecular Biotechnology session, V Annual Symposium of Doctoral Program in Molecular and Environmental Biology (PDBMA). B1 CP2 Auditorium – University of Minho, Braga, Portugal, Oct 10, 2019

9. Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. ”Characterization of Cyberlindnera jadinii carboxylate permeases by heterologous expression in Saccharomyces cerevisiae”, selected for Poster-Flash Presentation, 8th Congress of European Microbiologists - FEMS2019. Glasgow, Scotland, July 7-11, 2019 (FEMS Grant)

8. Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. “Identification of novel carboxylate transporters encoded in the Cyberlindnera jadinii transportome by heterologous expression in Saccharomyces cerevisiae”, selected for Poster-Flash Presentation, 7th Conference on Physiology of Yeast and Filamentous Fungi. University of Milano-Bicocca, Milan, Italy, June 24-27, 2019 (FEMS Grant)

7. Sousa-Silva, M., Simões, M., Melo, L., Machado, I. “Pseudomonas ?uorescens tolerance to benzyldimethyldodecyl ammonium chloride: altered phenotype and cross-resistance.”, Oral Communication, III International Caparica Conference in Antibiotic Resistance – IC2AR. Caparica, Portugal, June 10-13, 2019

6. Maria Sousa-Silva, David Ribas, Margarida Casal, Isabel Soares-Silva. “Functional characterization of Cyberlindnera jadinii carboxylate transporters in Saccharomyces cerevisiae”, Oral Communication, Annual Meeting 2018 – Portuguese Society of Genetics. I3S, Porto, Portugal, June 14-15, 2018

5. Maria Sousa-Silva, Margarida Casal, Isabel Soares-Silva. “Identification and functional characterization of novel plasma membrane carboxylate transporters”, Oral Communication, 3rd Joint BIOTECnico and AEM PhD Programs Workshop. Lisbon, Portugal, June 11-12, 2018

4. Maria Sousa-Silva, David Ribas, Ricardo Franco-Duarte, Célia Pais, Margarida Casal, Isabel Soares Silva. “Exploring organic acid producer microorganisms – Identification, morpho- and physiological characterization of wild yeast strains.”, Oral Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden". Braga, Portugal, June 8-9, 2018

3. Sousa-Silva, M., Casal, M, Soares-Silva, I. “Project: Identification and functional characterization of novel plasma membrane carboxylate transporters”, Oral Communication, 2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA). Braga, Portugal, Sept 25-27, 2017

2. Maria Sousa-Silva, Margarida Casal, Isabel Soares-Silva. “Characterization of novel membrane transporters for biotechnological applications”, Oral Communication, 2nd JOINT BIOTECnico AND AEM PhD PROGRAMS WORKSHOP. Lisbon, Portugal, Feb 15-16, 2017

1. Sousa-Silva, M., Melo, L., Machado, I. “Adaptive resistance to BDMDAC and cross-resistance to other antimicrobial agents”, Pitch Communication, 7th Edition of the Annual Symposium on Bioengineering in FEUP. Porto, Portugal, Apr 22-23, 2016

 

Poster Communications:

12. Sousa-Silva, M., Vieira, D., Ribas, D., Soares, P., Casal, M., Soares-Silva, I. “Exploring the Cyberlindnera jadinii transportome for the identification of novel carboxylate transporters”, Poster Communication in session II of Molecular Microbiology and Microbial Physiology, Microbiotec 19’   Congress of Microbiology and Biotechnology. University of Coimbra, Portugal, Dec 5-7, 2019

11. Senra, E., Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. ”Characterization of novel plasma membrane carboxylate transporters from non-conventional yeasts”, Poster Communication in session II of Molecular Microbiology and Microbial Physiology, Microbiotec 19’ – Congress of Microbiology and Biotechnology. University of Coimbra, Portugal, Dec 5-7, 2019

10. Sousa-Silva, M., Ribas, D., Franco-Duarte, R., Pais, C., Casal, M., Soares-Silva, I. “Screening of non Saccharomyces wild yeasts for biotechnological applications”, Poster Communication in session I of Environmental Microbiology and Biotechnology, Microbiotec 19’ – Congress of Microbiology and Biotechnology. University of Coimbra, Portugal, Dec 5-7, 2019

9. Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. “Characterization of Cyberlindnera jadinii carboxylate transporters by heterologous expression in Saccharomyces cerevisiae”, Poster Communication, 1st Conference on Emerging Applications of Microbes. KU Leuven, Leuven, Belgium, June 3-4, 2019

8. Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. “The Cyberlindnera jadinii carboxylate transporters Ady2 and Jen1 homologs are functional in Saccharomyces cerevisiae”, Poster Communication, Annual Symposium of Doctoral Program in Molecular and Environmental Biology (PDBMA). IB-S Auditorium, Braga, Portugal, Oct 25, 2018

7. David Ribas, Isabel Soares-Silva, Daniel Vieira, Maria Sousa-Silva, Joana Sá-Pessoa, João Azevedo-Silva, Sandra Cristina Viegas, Cecília Maria Arraiano, Sandra Paiva, Pedro Soares, Margarida Casal. “The Ady2 “NPAPLGL(M/S)” motif is critical for acetate uptake and binding”, Poster Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden". Braga, Portugal, June 8-9, 2018

6. Maria Sousa-Silva, David Ribas, Margarida Casal, Isabel Soares-Silva. “Functional characterization of Cyberlindnera jadinii carboxylate transporters in Saccharomyces cerevisiae”, Poster Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau Van Uden". Braga, Portugal, June 8-9, 2018

5. David Ribas, Isabel Soares-Silva, Joana Sá-Pessoa, Maria Sousa-Silva, João Azevedo-Silva, Sandra Paiva, Margarida Casal. “Structural and Functional characterization of AceTr transporter family”. Poster communication, Microbiotec 17’ – Congress of Microbiology and Biotechnology in UCP. Porto, Portugal, Dec 7-9, 2017

4. Sousa-Silva, M., Ribas, D., Casal, M., Soares-Silva, I. “Heterologous expression of Candida utilis carboxylic acid transporters in Saccharomyces cerevisiae”, Poster Communication – XLI Jornadas Portuguesas de Genética. Aveiro, Portugal, Jun 8-9, 2017

3. Sousa-Silva, M., Casal, M., Soares-Silva, I. “Characterization of novel membrane transporters with biotechnological applications”, Poster Communication – 2nd JOINT BIOTECnico AND AEM PhD PROGRAMS WORKSHOP. Lisbon, Portugal, Feb 15-16, 2017

2. Sousa-Silva, M., Simões, M., Melo, L., Machado, I. “Adaptation of Pseudomonas fluorescens to benzyldimethyldodecyl ammonium chloride: implication in biofilm formation and cross resistance”, Poster Communication - ESGB/EPASG Annual International Meeting on Antimicrobial Resistance in Microbial Biofilms and Options for Treatment. Ghent, Belgium, Oct 5 7, 2016

1. Sousa-Silva, M., Melo, L., Machado, I. “Adaptive resistance in gram-negative bacteria to BDMDAC and cross-resistance to other antimicrobial agents”, Pitch and Poster Communication – 7th Edition of the annual Symposium on Bioengineering in FEUP. Porto, Portugal, Apr 22-23, 2016

 

 

Underlined: presenting author

 

General Conferences: 

DP_AEM Conference (IB-S), Margarida Archer, ITQB, UNLisboa. "How can structure help understanding function? The story of arabinofuranosyltransferase and its role in the intricate cell wall synthesis of mycobacteria". Sponsored by Sociedade Portuguesa de Biofísica. 27 September 2018, Braga

DP_AEM Conference (IB-S), Johan Thevelein, GlobalYeast & KU Leuven, “Polygenic analysis of complex traits for development of superior industrial yeast strains” 25 September 2018, Braga

INL SUMMIT 2017 - “Nanotechnology: The New Economy” Conference organized by INL - International Iberian Nanotechnology Laboratory in Braga, Theatro Circo, Portugal from 19 - 20 Oct 2017.

XIX National Congress of Biochemistry in Guimarães, integrated in the organizing committee, from the 8-10 Dec 2016.

 

Workshops: 

"Wast, spills and personal protective equipment", 15th March, 2017, Braga, Portugal

 

Outreach activities: 

- Coordination and moderation of the Pint of Science Portugal event (online version) – Special edition in which Portuguese scientists around the world were invited to spoke about their work by Zoom sessions broadcast in live-streaming into the different social networks platforms (7, 8 and 9 of Sept 2020, online version)

- Coordination of 3-days-project for public engagement connecting Pint of Science Braga with European Researchers’ Night (Marie Sklodowska-Curie Actions) in collaboration of School of Sciences from University of Braga (6, 13 and 20 of Sept 2019, in Barhaus, Setra and Letraria, Braga, Portugal)

- Volunteer at Wikipedian TaskForce linked to WikiProject Microbiology promoted by FEMS (Jan 2019-2020)

- Principal Coordinator of Pint of Science Braga, a Global Science Festival (Out 2018-present, Braga, Portugal)

- Tutor at Open Weekend 2018 organized at University of Minho representing Molecular Biotechnology lab regarding the Protein transporter’s engineering group (19-21 April 2018, CBMA - Braga, Portugal)

- Collaborator of European Researchers’ Night, Marie Sk?odowska-Curie Actions (29 Sept 2017, Salão dos Congregados - Braga, Portugal)

- Participation at “Semana da Ciência e Tecnologia” organized at University of Minho representing Molecular Biotechnology lab regarding the Protein transporter’s engineering group in their laboratory activities (24 Nov 2017, CBMA - Braga, Portugal)

- Orientation at practical activities of BSc students Helena Ribeiro (Biochemistry degree, 2016) and Emanuel Senra (Biotechnology degree, 2018/2019) and of the PhD student João Alves (orientation at initial stage of PhD, 2019/2020) developed within the scope of the research project.

- Summer School of Math: EVMAT 2009 - FCUP, 2009

- Semi-final of Chemistry + Olympics 2008 - FCUP, 2008

 

Training

Hands-on approach Workshop – DD-DeCaF: Data-driven cell factory design, at ITQB in Oeiras, 18-19 Sept 2018, Lisbon, Portugal

“Genomics, Metagenomics and Bioinformatics”, June 2017, University of Minho, Braga

“Fluorescence microscopy”, at IB-S in 26 April 2018, Braga, Portugal

Transferable Skills, by Dr. Cátia Marques at IB-S in 26 Sept 2018, Braga, Portugal

Communication Skills (course level 1), by Dr. Ana Salgado at 30 Sept 2016, Braga, Portugal

Communication Skills (course level 2), by Dr. Ana Salgado from Feb - July 2017, Braga, Portugal

Awards

FEBS short-term fellowship to support the visit at TU Delft in 2020, Delft, Netherlands.

FEMS Meeting Grant to support the attendance of early-career researchers to the 8th Congress of European Microbiologists - FEMS2019, 7-11 July, 2019, Glasgow, Scotland.

FEMS Meeting Grant to support the attendance of early-career researchers to the 7th Conference on Physiology of Yeast and Filamentous Fungi, 24-27 June, 2019, Milan, Italy.

NORTE2020 4 year-PhD Studentship (from October 2016 till September 2020) to conduct a PhD at University of Minho (Braga, Portugal).

Erasmus + fellowship to support research work at Université Paris Descartes (Université Paris Sorbonne Cité, Paris, France) from May till September 2016.

Erasmus fellowship to support research work at Ecole Centrale Paris (Centrale Supélec, Paris, France) from March till July 2014.