Maria Antónia
Silva
...
DP_AEM student
Centro de Biologia Molecular e Ambiental (CBMA)
Doctoral Program in Molecular and Environmental Biology (CBMA)
2016-2020
Isabel Soares Silva
Maria Antónia
0
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Phd Thesis

Identification and functional characterization of novel plasma membrane carboxylate transporters for biotechnological applications

Several carboxylic acids are among the top value added chemicals produced from biomass, as these building-blocks can be subsequently converted to a number of high-value bio-based chemicals or materials. Different strategies have been developed to improve the cell factory production of these acids; however the export of these molecules is still a major bottleneck. In our group the study of carboxylic acid transporters has been one of the major focuses of research for several years now. The characterization of these transporters is done both at the functional and molecular levels. Transporter proteins are characterized by heterologous expression in a Saccharomyces cerevisiae strain with no carrier-mediated carboxylate transport at the plasma membrane. In addition, structure-function studies are conducted to unravel the mechanisms of substrate recognition and specificity of these proteins. As a final stage, developed metabolic engineered S. cerevisiae robust strains are going to be set growing in bioreator cultivations for otimization of microbial production of desired carboxylic acids.

Publication and Meetings

ORCID: 0000-0003-1622-4888

Linkedin: linkedin.com/in/maria-antónia-silva-64847563

1st Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 26-30 Sept 2016

 

2nd Joint BIOTECnico and AEM PhD Programs Workshop, Instituto Superior Técnico, Lisboa, Portugal, 15-16 Feb 2017

 

2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA), Braga, Portugal 25-29 Sep 2017

Oral Communications:

 

6. Maria Sousa-Silva, David Ribas, Margarida Casal, Isabel Soares-Silva. “Functional characterization of Cyberlindnera jadinii carboxylate transporters in Saccharomyces cerevisiae.”, Oral Communication, Annual Meeting 2018 – Portuguese Society of Genetics. I3S – Porto, Portugal, June 14-15, 2018

 

 

5. Maria Sousa-Silva, Margarida Casal, Isabel Soares-Silva. “Identification and functional characterization of novel plasma membrane carboxylate transporters”, Oral Communication, 3rd Joint BIOTECnico and AEM PhD Programs Workshop . Lisboa, Portugal, June 11-12 , 2018

 

 

4. Maria Sousa-Silva, David Ribas, Ricardo Franco-Duarte, Célia Pais, Margarida Casal, Isabel Soares-Silva. “Exploring organic acid producer microorganisms – Identification, morpho- and physiological characterization of wild yeast strains.”, Oral Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden". Braga, Portugal, June 8-9, 2018

 

 

3. Sousa-Silva M, Casal M, Soares-Silva I. “Project: Identification and functional characterization of novel plasma membrane carboxylate transporters”, Oral Communication, 2nd Joint Workshop of the PhD Programs in Applied and Environmental Microbiology (DP_AEM) and Molecular and Environmental Biology (PDBMA). Braga, Portugal, Sept 25-27, 2017

 

 

2. Maria Sousa-Silva, Margarida Casal, Isabel Soares-Silva. “Characterization of novel membrane transporters for biotechnological applications”, Oral Communication, 2nd JOINT BIOTECnico AND AEM PhD PROGRAMS WORKSHOP. Lisboa, Portugal, Feb 15-16, 2017

 

 

1. Sousa-Silva M, Melo L, Machado I. “Adaptive resistance to BDMDAC and cross-resistance to other antimicrobial agents”, Pitch Communication, 7th Edition of the annual Symposium on Bioengineering in FEUP. Porto, Portugal, Apr 22-23, 2016

 

 

 

 

Poster Communications:

 

7. David Ribas, Isabel Soares-Silva, Daniel Vieira, Maria Sousa-Silva, Joana Sá-Pessoa, João Azevedo-Silva, Sandra Cristina Viegas, Cecília Maria Arraiano, Sandra Paiva, Pedro Soares, Margarida Casal. “The Ady2 “NPAPLGL(M/S)” motif is critical for acetate uptake and binding”, Poster Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden". Braga, Portugal, June 8-9, 2018

 

 

6. Maria Sousa-Silva, David Ribas, Margarida Casal, Isabel Soares-Silva. “Functional characterization of Cyberlindnera jadinii carboxylate transporters in Saccharomyces cerevisiae.”, Poster Communication, XXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden". Braga, Portugal, June 8-9, 2018

 

 

5. David Ribas, Isabel Soares-Silva, Joana Sá-Pessoa, Maria Sousa-Silva, João Azevedo-Silva, Sandra Paiva, Margarida Casal. “Structural and Functional characterization of AceTr transporter family”, Poster communication, Microbiotec 17’ – Congress of Microbiology and Biotechnology in UCP. Porto, Portugal, Dec 7-9, 2017

 

 

4. Sousa-Silva M, Ribas D, Casal M, Soares-Silva I. “Heterologous expression of Candida utilis carboxylic acid transporters in Saccharomyces cerevisiae”, Poster Communication – XLI Jornadas Portuguesas de Genética. Aveiro, Portugal, Jun 8-9, 2017

 

 

3. Sousa-Silva M, Casal M, Soares-Silva I. “Characterization of novel membrane transporters with biotechnological applications”, Poster Communication – 2nd JOINT BIOTECnico AND AEM PhD PROGRAMS WORKSHOP. Lisboa, Portugal, Feb 15-16, 2017

 

XIX National Congress of Biochemistry in Guimarães, integrated in the organizing committee, from the 8-10 Dec 2016.


2. Sousa-Silva M
, Simões M, Melo L, Machado I. “Adaptation of Pseudomonas fluorescens to benzyldimethyldodecyl ammonium chloride: implication in biofilm formation and cross-resistance”, Poster Communication - ESGB/EPASG Annual International Meeting on Antimicrobial Resistance in Microbial Biofilms and Options for Treatment. Ghent, Belgium, Oct 5-7, 2016

 

 

1. Sousa-Silva M, Melo L, Machado I. “Adaptive resistance to BDMDAC and cross-resistance to other antimicrobial agents”, Pitch and Poster Communication – 7th Edition of the annual Symposium on Bioengineering in FEUP. Porto, Portugal, Apr 22-23, 2016

 

 

 

Underlinedpresenting author

Collaborator of “Noite Europeia dos Investigadores” (29 Sept 2017, Salão dos Congregados - Braga)

- Participation at “Semana da Ciência e Tecnologia” organized at University of Minho representing Molecular Biotechnology lab regarding the Protein transporter’s engineering group in their laboratory activities (24 Nov 2017, CBMA - Braga)

- Tutor at Open Weekend 2018 organized at University of Minho representing Molecular Biotechnology lab regarding the Protein transporter’s engineering group (19-21 April 2018, CBMA - Braga)

Training

“Genomics, Metagenomics and Bioinformatics”, 2017, University of Minho, Braga

Communication Skills (course level 1), by Dr. Ana Salgado, 30 September 2016, Braga

Communication Skills (course level 2), by Dr. Ana Salgado, February - July 2017, Braga